Crystal Structure of Human Cholesterol Sulfotransferase (SULT2B1b) in the Presence of Pregnenolone and 3′-Phosphoadenosine 5′-Phosphate
Crystal Structure of Human Cholesterol Sulfotransferase (SULT2B1b) in the Presence of Pregnenolone and 3′-Phosphoadenosine 5′-Phosphate
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孕烯醇酮和 3-磷酸腺苷 5-磷酸盐存在下人胆固醇磺基转移酶 (SULT2B1b) 的晶体结构
DOI：
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发表时间：
2003
影响因子：
4.8
通讯作者：
L. Pedersen
中科院分区：
文献类型：
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作者：
K.A.Bunding Lee;H. Fuda;Y. C. Lee;M. Negishi;C. Strott;L. Pedersen
The gene for human hydroxysteroid sulfotransferase (SULT2B1) encodes two peptides, SULT2B1a and SULT2B1b, that differ only at their amino termini. SULT2B1b has a predilection for cholesterol but is also capable of sulfonating pregnenolone, whereas SULT2B1a preferentially sulfonates pregnenolone and only minimally sulfonates cholesterol. We have determined the crystal structure of SULT2B1a and SULT2B1b bound to the substrate donor product 3′-phosphoadenosine 5′-phosphate at 2.9 and 2.4 Å, respectively, as well as SULT2B1b in the presence of the acceptor substrate pregnenolone at 2.3 Å. These structures reveal a different catalytic binding orientation for the substrate from a previously determined structure of hydroxysteroid sulfotransferase (SULT2A1) binding dehydroepiandrosterone. In addition, the amino-terminal helix comprising residues Asp19 to Lys26, which determines the specificity difference between the SULT2B1 isoforms, becomes ordered upon pregnenolone binding, covering the substrate binding pocket.
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影响因子：
4.8
作者：
C. Shimizu;H. Fuda;H. Yanai;C. Strott
通讯作者：
C. Shimizu;H. Fuda;H. Yanai;C. Strott
影响因子：
4.4
作者：
Her, C;Wood, TC;Weinshilboum, RM
通讯作者：
Weinshilboum, RM
神经类固醇和行为。
DOI：
10.1016/s0074-7742(01)46067-3
发表时间：
2001
期刊：
International review of neurobiology.
影响因子：
--
作者：
Engel,SR;Grant,KA
通讯作者：
Grant,KA
硫酸胆固醇通过增加 Fra-1、Fra-2 和 Jun D 来刺激角质形成细胞中的外皮蛋白转录。
影响因子：
6.5
作者：
Hanley,K;Wood,L;Ng,DC;He,SS;Lau,P;Moser,A;Elias,PM;Bikle,DD;Williams,ML;Feingold,KR
通讯作者：
Feingold,KR
神经类固醇的生物合成及其合成的调节。
DOI：
10.1016/s0074-7742(01)46058-2
发表时间：
2001
期刊：
International review of neurobiology.
影响因子：
--
作者：
Mellon,SH;Vaudry,H
通讯作者：
Vaudry,H