Antioxidant and antimicrobial activity of camel milk casein hydrolysates and its fractions
Antioxidant and antimicrobial activity of camel milk casein hydrolysates and its fractions
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DOI：
10.1016/j.smallrumres.2016.05.002
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发表时间：
2016-06-01
影响因子：
1.8
通讯作者：
Kumar, Pavan
中科院分区：
文献类型：
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作者：
Kumar, Devendra;Chatli, Manish Kumar;Kumar, Pavan
对 Alcalase、α-胰凝乳蛋白酶和木瓜蛋白酶水解的骆驼奶酪蛋白进行超滤，并评估不同组分的抗氧化和抗菌活性。分析酪蛋白水解产物级分的抗氧化活性，即。 2,2'-联氮基-双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）、2,2'-二苯基-1-三硝基苯肼（DPPH）和三价铁还原抗氧化能力测定（FRAP），以及抑菌圈测定的抗菌活性。在 ABTS、DPPH 和 FRAP 测定中，整个水解产物的抑制活性显着较高 (P < 0.05)，而所有三种水解产物的 F2 (1-5 kDa)、F3 (5-10 kDa) 级分具有相对较高的抗氧化活性。还观察到α-胰凝乳蛋白酶可以产生具有较高抗氧化活性的蛋白质水解产物和级分。与部分水解产物相比，整个水解产物的抗菌活性（抑制区；mm）也更高；然而，在不同的级分中，抑菌区几乎是可比的。还观察到Alcalase和α-胰凝乳蛋白酶可以产生具有更高抗菌活性的肽。结果表明，骆驼奶酪蛋白水解产物可以进行分离以获得特定分子量的肽，然而，对于食品应用或供人类直接食用，使用全水解产物在其功能和生产成本方面可能更有利。 (C) 2016 Elsevier B.V. 保留所有权利。
Camel milk casein hydrolysates by Alcalase, alpha-Chymotrypsin and Papain were ultra-filtered and different fractions were assessed for antioxidant and antimicrobial activity. The casein hydrolysate fractions were analyzed for antioxidant activities viz. 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS), 2,2'-diphenyl-1-picrylhydrazyl (DPPH) and Ferric reducing antioxidant power assay (FRAP), and antimicrobial activity by inhibition zone assay. In ABTS, DPPH and FRAP assay, the inhibition activity was recorded significantly (P < 0.05) higher for whole hydrolysares whereas among fractions F2 (1-5 kDa), F3 (5-10 kDa) of all the three hydrolysates had comperatively higher antioxidant activity. It was also observed that alpha- Chymotrypsin could produce protein hydrolysates and fractions with higher antioxidant activities. The antimicrobial activity (zone of inhibition; mm) was also recorded higher for whole hydrolysates as compared to their fractions; however, among different fractions the inhibition zones were almost comparable. It was also observed that Alcalase and alpha-Chymotrypsin could produce paptides with higher antimicrobial activity. The results suggested that camel milk casein hydrolysates could be fractioned to get specific molecular weight peptides, however, for food application or for direct human consumption, use of whole hydrolysates could be more beneficial with regards to its functionalities and cost of production. (C) 2016 Elsevier B.V. All rights reserved.