Immunoreactive forms of luteinizing hormone-releasing hormone in the brains of aging rats exhibiting persistent vaginal estrus.
Immunoreactive forms of luteinizing hormone-releasing hormone in the brains of aging rats exhibiting persistent vaginal estrus.
复制标题
表现出持续阴道发情的衰老大鼠大脑中黄体生成素释放激素的免疫反应形式。
DOI：
10.1095/biolreprod31.2.343
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发表时间：
1984
影响因子：
3.6
通讯作者：
Bridges,RS
中科院分区：
文献类型：
--
作者：
Rubin,BS;King,JC;Bridges,RS
采用免疫细胞化学（ICC）技术对持续阴道发情（PVE）的中年雌性大鼠的促黄体生成素-释放激素（LHRH）系统进行了检测。三种LHRH抗血清（A-R419, A-R744, A-R422；由A. Arimura博士慷慨提供）针对LHRH分子的不同部分，用于比较衰老和年轻雌性大鼠神经元中存在的下丘脑肽的免疫反应分子部分。在年轻循环动物神经元中检测到的十肽的免疫反应形式存在于PVE雌性LHRH神经元的相同区域。免疫反应性LHRH核外核和纤维在中年不排卵雌性和年轻循环雌性的基底前脑的分布没有区别。此外，在光镜下，年轻和衰老雌性LHRH神经元的大体形态没有明显差异。特别重要的是成熟十肽的生理活性形式在中隆起末端区域的明显存在。这些数据使我们提出，不能从神经血管末梢释放足够数量的LHRH，而不是缺乏这种下丘脑十肽的可用性，导致衰老雌性大鼠的生殖能力下降。
The luteinizing hormone-releasing hormone (LHRH) system in middle-aged female rats exhibiting persistent vaginal estrus (PVE) was examined using immunocytochemical (ICC) techniques. Three LHRH antisera (A-R419, A-R744, A-R422; generously supplied by Dr. A. Arimura) directed toward different portions of the LHRH molecule were utilized to compare the immunoreactive molecular moieties of this hypothalamic peptide present in neurons of aging and young female rats. The immunoreactive forms of the decapeptide detected within neurons of young cycling animals were present within the same regions of the LHRH neurons of PVE females. Immunoreactive LHRH perikarya and fibers in the middle-aged anovulatory females did not differ in their distribution in the basal forebrain from those of young cycling females. Furthermore, no gross morphological differences were apparent at the light microscopic level in LHRH neurons from young and aging females. Of particular importance was the apparent presence of the physiologically active form of the mature decapeptide in terminal regions of the median eminence. These data lead us to propose that failure to release adequate amounts of LHRH from neurovascular terminals and not the lack of availability of this hypothalamic decapeptide contributes to reproductive decline in aging female rats.