Dietary Supplementation With Nonfermentable Fiber Alters the Gut Microbiota and Confers Protection in Murine Models of Sepsis.
Dietary Supplementation With Nonfermentable Fiber Alters the Gut Microbiota and Confers Protection in Murine Models of Sepsis.
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DOI：
10.1097/ccm.0000000000002291
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发表时间：
2017-05
影响因子：
8.8
通讯作者：
Aneja RK
中科院分区：
文献类型：
--
作者：
Morowitz MJ;Di Caro V;Pang D;Cummings J;Firek B;Rogers MB;Ranganathan S;Clark RSB;Aneja RK
微生物变化和全身炎症之间的联系在慢性疾病中已经被证明，但对急性炎症期间的这些相互作用知之甚少。本研究在两种脓毒症小鼠模型中，研究了饮食中添加纤维素(一种不可发酵的纤维)对肠道微生物区系、炎症标志物和存活率的影响。前瞻性实验研究。大学实验室。对象：6周龄雄性C57BL/6野生型小鼠。将小鼠分为低纤维(LF)、正常纤维(NF)或高纤维(HF)饮食，分别给予或不给予抗生素，然后通过盲肠结扎穿孔(CLP)或注射内毒素造成脓毒症。在饮食干预前后采集粪便样本进行微生物区系分析。与接受LF或NF饮食的小鼠相比，接受HF饮食的小鼠在CLP后表现出更高的存活率。生存期的改善与降低血清促炎细胞因子浓度、减少中性粒细胞在肺部的渗透以及减轻肝脏炎症有关。HF饮食也增加了内毒素注射后的存活率。对每个样本的细菌16S rRNA基因序列进行扩增、测序和分析。纤维补充剂增加了阿克曼草属和乳螺科的相对丰度，这两个类群通常与代谢健康有关。给HF饮食的小鼠注射抗生素，否定了阿克曼氏菌物种的丰富和CLP后的生存益处。在脓毒症的小鼠模型中，补充纤维素的饮食提供了微生物介导的生存优势。更好地了解饮食、微生物区系和全身疾病之间的联系，可能会为脓毒症患者带来新的治疗策略。
Links between microbial alterations and systemic inflammation have been demonstrated in chronic disease, but little is known about these interactions during acute inflammation. This study investigates the effect of dietary supplementation with cellulose, a nonfermentable fiber, on the gut microbiota, inflammatory markers, and survival in two murine models of sepsis. Prospective experimental study. University laboratory. Subjects: 6 week old male C57BL/6 wild-type mice. Mice were assigned to low-fiber (LF), normal-fiber (NF), or high-fiber (HF) diets with or without antibiotics for two weeks and then subjected to sepsis by cecal ligation and puncture (CLP) or endotoxin injection. Fecal samples were collected for microbiota analyses before and after dietary interventions. Mice that received a HF diet demonstrated increased survival after CLP relative to mice receiving LF or NF diets. The survival benefit was associated with decreased serum concentration of pro-inflammatory cytokines, reduced neutrophil infiltration in the lungs, and diminished hepatic inflammation. The HF diet also increased survival after endotoxin injection. Bacterial 16S rRNA gene sequences from each sample were amplified, sequenced, and analyzed. Fiber supplementation yielded an increase in relative abundance of the genera Akkermansia and Lachnospiraceae, taxa commonly associated with metabolic health. Administration of antibiotics to mice on the HF diet negated the enrichment of Akkermansia species and the survival benefit after CLP. Dietary supplementation with cellulose offers a microbe-mediated survival advantage in murine models of sepsis. Improved understanding of the link between diet, the microbiota, and systemic illness may yield new therapeutic strategies for patients with sepsis.