Systematic investigation of changes in oxidized cerebral cytochrome c oxidase concentration during frontal lobe activation in healthy adults
Systematic investigation of changes in oxidized cerebral cytochrome c oxidase concentration during frontal lobe activation in healthy adults
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DOI：
10.1364/boe.3.002550
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发表时间：
2012-10-01
影响因子：
3.4
通讯作者：
Elwell, Clare E.
中科院分区：
文献类型：
--
作者：
Kolyva, Christina;Tachtsidis, Ilias;Elwell, Clare E.
使用经颅近红外光谱（NIRS）测量;在健康成人功能激活期间，脑细胞色素c氧化酶（Delta[oxCCO]）氧化还原状态的变化受到仪器和算法问题的阻碍。本研究报告了在这种情况下测量的Delta[oxCCO]反应，并调查了该测量的可能混杂因素。连续额叶近红外光谱测量收集11名健康志愿者在6分钟的字谜解决任务，使用混合光学光谱仪（pHOS），结合多距离频率和宽带组件。仅对显示与功能激活一致的血流动力学反应的数据集进行Delta[oxCCO]反应询问。同时还提供了系统监测数据。系统研究了对Delta[oxCCO]响应的可能影响，以下因素无影响：1）选择用于拟合测量的衰减光谱的波长范围; 2）恒定或测量（实时pHOS）的差分光程因子; 3）功能激活期间的全身血液动力学变化; 4）功能激活期间的光散射变化。在存在功能激活的情况下测量的Delta[oxCCO]反应是异质的，大多数受试者显示氧化显著增加，但其他受试者则减少。我们得出结论，Delta[oxCCO]反应的异质性是生理性的，不是由测量中的混杂因素引起的。(C)2012美国光学学会
Using transcranial near-infrared spectroscopy (NIRS) to measure ;changes in the redox state of cerebral cytochrome c oxidase (Delta[oxCCO]) during functional activation in healthy adults is hampered by instrumentation and algorithm issues. This study reports the Delta[oxCCO] response measured in such a setting and investigates possible confounders of this measurement. Continuous frontal lobe NIRS measurements were collected from 11 healthy volunteers during a 6-minute anagram-solving task, using a hybrid optical spectrometer (pHOS) that combines multi-distance frequency and broadband components. Only data sets showing a hemodynamic response consistent with functional activation were interrogated for a Delta[oxCCO] response. Simultaneous systemic monitoring data were also available. Possible influences on the Delta[oxCCO] response were systematically investigated and there was no effect of: 1) wavelength range chosen for fitting the measured attenuation spectra; 2) constant or measured, with the pHOS in real-time, differential pathlength factor; 3) systemic hemodynamic changes during functional activation; 4) changes in optical scattering during functional activation. The Delta[oxCCO] response measured in the presence of functional activation was heterogeneous, with the majority of subjects showing significant increase in oxidation, but others having a decrease. We conclude that the heterogeneity in the Delta[oxCCO] response is physiological and not induced by confounding factors in the measurements. (C) 2012 Optical Society of America