Characterization of yeast artificial chromosomes containing interleukin genes on human chromosome 5.
Characterization of yeast artificial chromosomes containing interleukin genes on human chromosome 5.
复制标题
人类 5 号染色体上含有白细胞介素基因的酵母人工染色体的表征。
DOI：
10.1159/000133417
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Westbrook,CA
中科院分区：
文献类型：
--
作者：
Stock,W;Chandrasekharappa,SC;Neuman,WL;LeBeau,MM;Brownstein,BH;Westbrook,CA
为了更好地了解白细胞介素基因，IL 4和IL 5的组织和连锁，我们制备了含有IL 5的5个酵母人工染色体（YAC）的长距离限制性内切酶图谱。我们确定IL 4和IL 5在100-170 kb内，并且这些基因周围的区域包含几个富含GC的区域。荧光原位染色体分析表明，5个YAC克隆中的3个含有源自多个人类染色体的非连续基因组序列。
To understand better the organization and linkage of the interleukin genes, IL4 and IL5, we prepared long-range restriction maps of five yeast artificial chromosomes (YACs) containing IL5. We determined that IL4 and IL5 are within 100–170 kb, and that the regions surrounding these genes contain several GC-rich areas. Fluorescence in situ chromosomal analysis demonstrated that three of the five YAC clones contain non-contiguous genomic sequences originating from multiple human chromosomes.