Bone Piezoelectricity-Mimicking Nanocomposite Membranes Enhance Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells by Amplifying Cell Adhesion and Actin Cytoskeleton
Bone Piezoelectricity-Mimicking Nanocomposite Membranes Enhance Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells by Amplifying Cell Adhesion and Actin Cytoskeleton
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模拟骨压电纳米复合膜通过增强细胞粘附和肌动蛋白细胞骨架来增强骨髓间充质干细胞的成骨分化
DOI：
10.1166/jbn.2021.3090
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发表时间：
2021
影响因子：
2.9
通讯作者：
Xuehui Zhang
中科院分区：
文献类型：
--
作者：
Xiaowen Sun;Yunyang Bai;Xiaona Zheng;Xiaochan Li;Yingying Zhou;Yijun Wang;Boon Chin Heng;Xuehui Zhang
铁电生物材料已被广泛研究，并证明通过模拟骨组织的固有电特性来增强骨生成。尽管如此，基本的生物过程仍然没有得到很好的理解。因此，本研究探讨了骨压电模拟钛酸钡/聚（偏氟乙烯-三氟乙烯）纳米复合膜（BTO纳米复合膜）促进骨髓间充质干细胞（BMSCs）成骨的潜在生物学过程。结果表明，可控电晕极化后的纳米复合膜的压电系数（d33）与天然骨的压电系数（d33）相似，并表现出高度稳定的压电性能和集中的表面电位。这些纳米复合膜显著增强了BMSCs的粘附和铺展，表现为成熟的局灶性粘附的数量和面积增加。此外，纳米复合膜显著促进整合素受体基因（α1、α5和β3）的表达，这反过来又增强了BMSCs的成骨作用，如上调碱性磷酸酶（ALP）和骨形态发生蛋白2（BMP 2）表达水平所示。进一步研究发现，粘着斑激酶（FAK）-细胞外信号调节激酶1/2（ERK 1/2）信号转导轴可能参与了极化纳米复合膜诱导成骨的生物学过程。本研究为更好地理解压电或铁电生物材料促进骨生成的生物学过程提供了有益的见解。
Ferroelectric biomaterials have been widely investigated and demonstrated to enhance osteogenesis by simulating the inherent electrical properties of bone tissues. Nevertheless, the underlying biological processes are still not wellunderstood. Hence, this study investigated the underlying biological processes by which bone piezoelectricity-mimicking barium titanate/poly(vinylidene fluoride-trifluoroethylene) nanocomposite membranes (BTO nanocomposite membranes) promote osteogenesis of Bone Marrow Mesenchymal Stem Cells (BMSCs). Ourresults revealed that the piezoelectric coefficient (d 33) of nanocomposite membranes aftercontrolled corona poling was similar to that of native bone, and exhibited highly-stable piezoelectrical properties and concentrated surface electrical potential. These nanocomposite membranes significantly enhanced the adhesion and spreading of BMSCs, which was manifested as increased number and area of mature focal adhesions. Furthermore, the nanocomposite membranes significantly promoted the expression of integrin receptors genes (α1, α5 andβ3), which in turn enhanced osteogenesis of BMSCs, as manifested by upregulated Alkaline Phosphatase (ALP) and Bone Morphogenetic Protein 2 (BMP2) expression levels. Further investigations found that the Focal Adhesion Kinase (FAK)-Extracellular Signal-Regulated Kinase1/2 (ERK 1/2) signaling axis may be involved in the biological process of polarized nanocomposite membrane-induced osteogenesis. This study thus provides useful insights for betterunderstanding of the biological processes by which piezoelectric or ferroelectric biomaterials promote osteogenesis.