SEQUENCE OF THE NOTCH LOCUS OF DROSOPHILA-MELANOGASTER - RELATIONSHIP OF THE ENCODED PROTEIN TO MAMMALIAN CLOTTING AND GROWTH-FACTORS
SEQUENCE OF THE NOTCH LOCUS OF DROSOPHILA-MELANOGASTER - RELATIONSHIP OF THE ENCODED PROTEIN TO MAMMALIAN CLOTTING AND GROWTH-FACTORS
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DOI：
10.1128/mcb.6.9.3094
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发表时间：
1986-09-01
影响因子：
5.3
通讯作者：
YOUNG, MW
中科院分区：
文献类型：
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作者：
KIDD, S;KELLEY, MR;YOUNG, MW
Notch基因座对果蝇外胚层的正确分化至关重要。Notch对应于一个37千碱基的转录单位，编码一个主要的10.4千碱基多聚腺苷酸化RNA。该转录单位的DNA序列，除了两个最大的插入序列的部分。还从三个Notch cDNA克隆中获得DNA序列，从而可以对基因的5“和3”末端进行作图，并确定九个RNAa编码区的结构和位置。主要的Notch转录物编码2，703个氨基酸的蛋白质。该蛋白可能与细胞表面相关，并携带由36个富含半胱氨酸的重复单元组成的胞外结构域，每个单元约38个氨基酸。该基因似乎是通过重复串联复制编码38个氨基酸长的蛋白质片段的DNA，然后插入插入序列而进化的。这些重复的蛋白片段与哺乳动物凝血因子IX和X的部分以及秀丽隐杆线虫发育基因lin-12的产物非常同源。它们也类似于哺乳动物生长激素，典型的是表皮生长因子。
The Notch locus is essential for proper differentiation of the ectoderm in Drosophila melanogaster. Notch corresponds to a 37-kilobase transcription unit that codes for a major 10.4-kilobase polyadenylated RNA. The DNA sequence of this transcription unit is presented, except for portions of the two largest intervening sequences. DNA sequences also were obtained from three Notch cDNA clones, allowing the 5'' and 3'' ends of the gene to be mapped, and the structures and locations of nine RNAa coding regions to be determined. The major Notch transcript encodes a protein of 2,703 amino acids. The protein is probably associated with cell surfaces and carries an extracellular domain composed of 36 cysteine-rich repeating units, each of about 38 amino acids. The gene appears to have evolved by repeated tandem duplications of the DNA coding for the 38-amino-acid-long protein segments, followed by insertion of intervening sequences. These repeating protein segments are quite homologous to portions of mammalian clotting factors IX and X and to the product of the Caenorhabditis elegans developmental gene lin-12. They are also similar to mammalian growth hormones, typified by epidermal growth factor.