Whole-Cell Impedance Analysis for Highly and Poorly Metastatic Cancer Cells
Whole-Cell Impedance Analysis for Highly and Poorly Metastatic Cancer Cells
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DOI：
10.1109/jmems.2009.2021821
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发表时间：
2009-08-01
影响因子：
2.7
通讯作者：
Han, Arum
中科院分区：
文献类型：
--
作者：
Cho, Younghak;Kim, Hyun Soo;Han, Arum
微电阻抗谱（μ EIS）系统已经开发和实施，以分析高和低转移性头颈癌（HNC）细胞系与单细胞分辨率。该微系统具有16个阻抗分析位点的阵列，每个位点都能够捕获单个细胞并分析其全细胞电阻抗谱。该μ EIS系统用于获得低转移性HNC细胞系686 LN和高转移性HNC细胞系686 LN-M4 e在40 Hz-10 MHz频率范围内的电阻抗谱。与686 LN-M4 e电池相比，686 LN电池在50 kHz和2 MHz之间的频率下具有更高的阻抗相位。该结果表明，HNC细胞的转移状态可以使用开发的μ EIS系统来区分。该系统有望成为未来检测和定量不同肿瘤阶段癌细胞的有力工具。
A micro electrical impedance spectroscopy (mu EIS) system has been developed and implemented to analyze highly and poorly metastatic head and neck cancer (HNC) cell lines with single-cell resolution. The microsystem has arrays of 16 impedance analysis sites, each of which is capable of capturing a single cell and analyzing its whole-cell electrical impedance spectrum. This mu EIS system was used to obtain the electrical impedance spectra of the poorly metastatic HNC cell line 686LN and the highly metastatic HNC cell line 686LN-M4e over a frequency range of 40 Hz-10 MHz. The 686LN cells had higher impedance phase compared to that of 686LN-M4e cells at frequencies between 50 kHz and 2 MHz. This result demonstrates that the metastatic state of HNC cells can be distinguished using the developed mu EIS system. This system is expected to serve as a powerful tool for future detection and quantification of cancer cells from various tumor stages.