Broccoli microgreens juice reduces body weight by enhancing insulin sensitivity and modulating gut microbiota in high-fat diet-induced C57BL/6J obese mice
Broccoli microgreens juice reduces body weight by enhancing insulin sensitivity and modulating gut microbiota in high-fat diet-induced C57BL/6J obese mice
复制标题
西兰花微绿汁通过增强胰岛素敏感性和调节高脂肪饮食诱导的 C57BL/6J 肥胖小鼠的肠道微生物群来减轻体重
DOI：
10.1007/s00394-021-02553-9
复制
发表时间：
2021-04-17
影响因子：
5
通讯作者：
Lu, Yingjian
中科院分区：
文献类型：
--
作者：
Li, Xiangfei;Tian, Shuhua;Lu, Yingjian
目的本研究旨在探讨西兰花微绿汁（BMJ）对C57BL/6J小鼠肥胖发展的保护作用。方法采用高脂饮食（HFD）诱导的肥胖模型小鼠，用BMJ灌胃10周。以 300 mg/(kg bw)/d 灌胃 Melbine，作为阳性对照组。结果补充 BMJ 显着降低了 HFD 喂养小鼠的白色脂肪组织 (WAT) 质量、体重和脂肪细胞大小，并增加了水摄入量。此外，它还能改善糖耐量，降低胰岛素水平和HOMA-IR值，减轻胰岛素抵抗。与HFD组相比，补充BMJ在门水平上显着增加了Bacteroidetes的相对丰度，降低了Firmicutes与Bacteroidetes的比例，并在种水平上富集了Bacteroides_acidifaciens。肠道菌群组成的这些变化与短链脂肪酸（SCFAs）的产生有关，并降低了LPS水平，并具有明显的抗炎作用。结论这些研究结果表明，BMJ对饮食诱导的肥胖的保护作用可能涉及肠道菌群-SCFAs-LPS-炎症轴。此外，BMJ还能增强肝脏抗氧化能力，减少肝脏脂肪堆积。因此，这些结果支持 BMJ 作为一种新型肥胖功能食品，因为它对 HFD 诱导的小鼠肥胖具有相反作用。
PurposeThis study aimed to explore the protective effect of broccoli microgreens juice (BMJ) during C57BL/6J mice obesity development.MethodsThe obese model mice, induced by feeding high-fat diet (HFD), were treated with BMJ by gavage for 10 weeks. Melbine was gavaged at 300 mg/(kg bw)/d, as a positive control group.ResultsBMJ supplementation significantly reduced white adipose tissues (WAT) mass, the body weight and adipocyte size, and increased water intake in HFD-fed mice. Moreover, it improved glucose tolerance, reduced insulin level and HOMA-IR value, and alleviated insulin resistance. Compared with the HFD group, BMJ supplementation significantly increased the relative abundance ofBacteroidetesand decreased the ratio ofFirmicutestoBacteroidetesat the phylum level, and enrichedBacteroides_acidifaciensat the species level. These changes in the composition of gut microbiota are associated with the production of short-chain fatty acids (SCFAs), and reduced LPS levels, and had an obvious anti-inflammatory effect.ConclusionsThese findings suggested that the protective effects of BMJ on diet-induced obesity may be involved in gut microbiota–SCFAs–LPS–inflammatory axis. In addition, BMJ can enhance liver antioxidant capacity and reduce liver fat accumulation. Consequently, these results sustain BMJ as a novel functional food for obesity, on the basis of its opposing effects on HFD-induced obesity in mice.