HIV-specific cytotoxic T lymphocyte precursors exist in a CD28-CD8+T cell subset and increase with loss of CD4 T cells
HIV-specific cytotoxic T lymphocyte precursors exist in a CD28-CD8+T cell subset and increase with loss of CD4 T cells
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DOI：
10.1097/00002030-199906180-00005
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发表时间：
1999-06-18
期刊：
影响因子：
3.8
通讯作者：
Rodgers, JR
中科院分区：
文献类型：
--
作者：
Lewis, DE;Yang, LX;Rodgers, JR
目的：为了确定是否CD 28-CD 8 + T细胞，在HIV感染过程中发展包含HIV特异性细胞毒性前体cells.Design：CD 8亚群从6个无症状的HIV阳性成人，不同程度的CD 4 T细胞的损失，通过缺陷细胞计数和HIV特异性前体细胞毒性T淋巴细胞的频率进行了测量。检测了三种CD 8 T细胞群：CD 28 + CD 57- T细胞、CD 28-CD 57 + T细胞（被认为是记忆细胞）和CD 28-CD 57- T细胞（功能未知）。2周后，对表达Gag/Pol的Cr-51标记的EB病毒转化的自体B细胞系或对照靶标进行细胞毒性T细胞测定。结果：CD 28+和CD 28-CD 57+细胞均含有相当数量的前体细胞（9-1720/10（6）CD 8 + T细胞）。然而，在研究的六个人中，有五个人的CD 28-CD 57-群体的前体较少。CD 4：CD 8比值> 1的患者中，CD 28阳性的HIV特异性细胞毒性T淋巴细胞前体细胞较多，而CD 1：CD 8比值< 1的患者中，CD 28-CD 57+前体细胞较多（r（2），0.68）。结论：在CD 28阳性和CD 28-CD 8+细胞中均存在记忆性HIV特异性细胞毒性T淋巴细胞前体细胞，但CD 28-CD 57-亚群较少。由于CD 28-CD 57+细胞是抗原驱动的，多样性有限，因此疾病进展期间CD 8 T细胞上CD 28的丢失可能会降低对新HIV突变的反应;这需要进一步检测。(C)1999年利平科特威廉姆斯&威尔金斯。
Objectives: To determine whether the CD28-CD8+ T cells that develop during HIV infection contain HIV-specific cytotoxic precursor cells.Design: CD8 subpopulations from six asymptomatic HIV-positive adults, with varying degrees of CD4 T cell loss, were sorted by flaw cytometry and HIV-specific precursor cytotoxic T lymphocyte frequencies were measured. Three populations of CD8 T cells were tested: CD28+CD57- T cells, CD28-CD57+ T cells (thought to be memory cells) and CD28-CD57- T cells (function unknown).Methods: Sorted CD8 subsets were stimulated with antigen presenting cells expressing HIV-1 Gag/Pol molecules. Cytotoxic T cell assays on Gag/Pol expressing Cr-51-labeled Epstein-Barr virus transformed autologous B cells lines or control targets were performed after 2 weeks. Specific lysis and precursor frequencies were calculated.Results: Both CD28 positive and CD28-CD57+ populations contained appreciable numbers of precursors (9-1720 per 10(6) CD8+ T cells). However, the CD28-CD57-population had fewer precursors in five out of six people studied. More CD28 positive HIV-specific cytotoxic T lymphocyte precursors were found in patients with CD4:CD8 ratios > 1, whereas more CD28-CD57+ precursors were found in patients whose CD 1 : CD8 ratios were < 1 (r(2), 0.68).Conclusions: Memory HIV-specific precursor cytotoxic T lymphocytes are found in both CD28 positive and CD28-CD8+ cells, however, a CD28-CD57-subpopulation had fewer. Because CD28-CD57+ cells are antigen-driven with limited diversity, the loss of CD28 on CD8 T cells during disease progression may reduce the response to new HIV mutations; this requires further testing. (C) 1999 Lippincott Williams & Wilkins.