Oestrogen receptor gene polymorphisms and ovarian stimulation for in-vitro fertilization
Oestrogen receptor gene polymorphisms and ovarian stimulation for in-vitro fertilization
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DOI：
10.1093/humrep/12.7.1430
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发表时间：
1997-07-01
影响因子：
6.1
通讯作者：
Lolis, DE
中科院分区：
文献类型：
--
作者：
Georgiou, I;Konstantelli, M;Lolis, DE
实验证据表明，缺乏雌激素受体（ESR）基因的小鼠不育，卵巢囊肿和卵泡停滞，在人类中，ESR基因的几个多态性和突变已被确定，在这项研究中，我们分析了一个常见的PvuII和罕见的BstUI多态性的ESR基因。对接受卵巢刺激进行体外受精（IVF）和胚胎移植的妇女和至少有一次妊娠的对照组的DNA样本进行分析。比较三种PvuII基因型之间的卵泡和卵母细胞的平均数量和卵泡与卵母细胞的平均比率，在连续两个周期中收获。与具有杂合或纯合PvuII多态性的组相比，在缺乏PvuII多态性的组中鉴定出显著较低的比率（P > 0.05和P > 0.01）。在一条染色体上同时具有PvuII和BstUI限制性酶切位点的罕见单倍型仅在IVF组中存在，在PvuII多态性纯合子患者中，IVF妊娠显著罕见我们的研究结果表明ESR的遗传变异性对卵巢对刺激的反应性判断卵泡的质量有一定的作用，也可能影响着床。
Experimental evidence has shown that mice lacking the oestrogen receptor (ESR) gene are infertile with cystic ovaries and follicular arrest, In humans, several polymorphisms and mutations in the ESR gene have been identified, In this study we have analysed a common PvuII and a rare BstUI polymorphism in the ESR gene. Analysis was carried out on DNA samples from women undergoing ovarian stimulation for in-vitro fertilization (IVF) and embryo transfer and controls having at least one pregnancy. Comparisons were done between the three PvuII genotypes, concerning the mean numbers of follicles and oocytes and the mean ratios of follicles to oocytes harvested in two consecutive cycles. Significantly lower ratios were identified in the group lacking the PvuII polymorphism, compared with the groups with heterozygous or homozygous PvuII polymorphisms (P > 0.05 and P > 0.01 respectively), The rare haplotype having both PvuII and BstUI restriction sites on one chromosome was present only in the IVF group, Pregnancies from IVF were significantly rarer in patients who were homozygous for the PvuII polymorphism (P > 0.05), Our results suggest that genetic variability in the ESR has a role in the quality of the ovarian follicles as judged by the ovarian response to stimulation and may also affect implantation.