Rtt109 acetylates histone H3 lysine 56 and functions in DNA replication
Rtt109 acetylates histone H3 lysine 56 and functions in DNA replication
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DOI：
10.1126/science.1133234
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发表时间：
2007-02-02
期刊：
影响因子：
56.9
通讯作者：
Zhang, Zhiguo
中科院分区：
文献类型：
--
作者：
Han, Junhong;Zhou, Hui;Zhang, Zhiguo
组蛋白H3赖氨酸56（H3-K56）的乙酰化发生在S期，缺乏H3-K56乙酰化的细胞对DNA损伤剂敏感。然而，组蛋白乙酰转移酶（HAT）催化全球H3-K56乙酰化还没有发现。缺乏Rtt 109或表达在保守的天冬氨酸残基处具有改变的rtt 109突变体的细胞失去H3-K56乙酰化，并表现出对遗传毒性剂的敏感性增加，以及自发染色体断裂水平升高。因此，Rtt 109与任何其他已知的HAT没有序列同源性，是一种独特的HAT，它使H3-K56乙酰化。
Acetylation of histone H3 lysine 56 (H3-K56) occurs in S phase, and cells lacking H3-K56 acetylation are sensitive to DNA-damaging agents. However, the histone acetyltransferase (HAT) that catalyzes global H3-K56 acetylation has not been found. Here we show that regulation of Ty1 transposition gene product 109 (Rtt109) is an H3-K56 HAT. Cells lacking Rtt109 or expressing rtt109 mutants with alterations at a conserved aspartate residue lose H3-K56 acetylation and exhibit increased sensitivity toward genotoxic agents, as well as elevated levels of spontaneous chromosome breaks. Thus, Rtt109, which shares no sequence homology with any other known HATs, is a unique HAT that acetylates H3-K56.