Terrimonas rubra sp. nov., isolated from a polluted farmland soil and emended description of the genus Terrimonas.
Terrimonas rubra sp. nov., isolated from a polluted farmland soil and emended description of the genus Terrimonas.
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DOI：
10.1099/ijs.0.036079-0
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发表时间：
2012-11
影响因子：
2.8
通讯作者：
Jing Zhang;Tao Gu;Yu Zhou-;Jie He;Liu-Qiang Zheng;Wenjun Li;Xing Huang;Shun-Peng Li
中科院分区：
文献类型：
--
作者：
Jing Zhang;Tao Gu;Yu Zhou-;Jie He;Liu-Qiang Zheng;Wenjun Li;Xing Huang;Shun-Peng Li
从我国受污染的农田土壤中分离到一株产橙红色色素的细菌，命名为M-8（T），并对其进行了多相分类研究。菌株M-8（T）革兰氏染色阴性，杆状，不运动，不产芽孢。生长发生在20-37 °C，pH 5.0-10.0和0-2%（w/v）NaCl下。基于16 S rRNA基因序列的系统发育分析表明，菌株M-8（T）属于Terrimonas属，菌株M-8（T）与Terrimonas属3个已知种的模式菌株Terrimonas ferrugenas KACC 11310（T）、Terrimonas aquatica LMG 24825（T）和Terrimonas lutea KACC 13047（T）的16 S rRNA基因序列相似性为97.1，96.3和95.3%。主要呼吸醌是甲基萘醌-7（MK-7），主要脂肪酸是异-C（15：0）、异-C（17：0）3-OH和总和特征3（包括C（16：1）ω 7 c和/或C（16：1）ω6c）。菌株M-8（T）的DNA G+C含量为47.0mol%。基于基因型和表型数据，菌株M-8（T）被认为是代表一个新的种属Terrimonas rubra sp. nov.，建议将其命名为Terrimonas rubra sp. nov.。模式菌株为M-8（T）（=CCTCC AB 2010401（T）=KCTC 23299（T））。此外，还提出了对Terrimonas属的一个修订描述。
A salmon-red-pigmented bacterial strain, designated M-8(T), was isolated from a polluted farmland soil sample in China and was characterized in a taxonomic study using a polyphasic approach. Strain M-8(T) was Gram-stain-negative, rod-shaped, non-motile and non-spore-forming. Growth occurred at 20-37 °C, at pH 5.0-10.0 and with 0-2% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain M-8(T) belonged to the genus Terrimonas.16S rRNA gene sequence similarity values between strain M-8(T) and the type strains of the three recognized species of the genus Terrimonas, Terrimonas ferruginea KACC 11310(T), Terrimonas aquatica LMG 24825(T) and Terrimonas lutea KACC 13047(T), were 97.1, 96.3 and 95.3%, respectively. The predominant respiratory quinone was menaquinone-7 (MK-7) and the major fatty acids were iso-C(15:0), iso-C(17:0) 3-OH and summed feature 3 (comprising C(16:1)ω7c and/or C(16:1)ω6c). The DNA G+C content of strain M-8(T) was 47.0 mol%. On the basis of genotypic and phenotypic data, strain M-8(T) is considered to represent a novel species of the genus Terrimonas, for which the name Terrimonas rubra sp. nov. is proposed. The type strain is M-8(T) (=CCTCC AB 2010401(T)=KCTC 23299(T)). An emended description of the genus Terrimonas is also presented.