Method to quantify cytokines and chemokines in mouse brain tissue using Bio-Plex multiplex immunoassays
Method to quantify cytokines and chemokines in mouse brain tissue using Bio-Plex multiplex immunoassays
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DOI：
10.1016/j.ymeth.2019.02.007
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发表时间：
2019-04-01
期刊：
影响因子：
4.8
通讯作者：
Oh, Kenneth
中科院分区：
文献类型：
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作者：
Manglani, Monica;Rua, Rejane;Oh, Kenneth
本方案描述了如何制备小鼠脑组织，以便使用基于多重微珠的免疫分析对多种炎症介质进行定量。重要的是要有能够从少量组织中定量多个分析物的方法。Bio-Plex是一种基于Luminex xMAP的多重珠状免疫分析技术，允许同时分析单个组织样本中的多达100种分析物。该方法已被广泛用于研究血浆和血清样本以及培养细胞和原代细胞中的分析物。在这里，我们描述了一种使用Bio-Plex Pro Mouse Chemokine Panel 33-Plex同时分析小鼠脑组织中33种不同的炎性细胞因子和趋化因子的方法。
This protocol describes how to prepare mouse brain tissue for quantification of multiple inflammatory mediators using a multiplex bead-based immunoassay. It is important to have methods that allow quantification of multiple analytes from small amounts of tissue. Bio-Plex is a Luminex xMAP-based multiplex bead-based immunoassay technology that permits simultaneous analysis of up to 100 analytes from a single tissue sample. This assay has been used extensively to investigate analytes in plasma and serum samples as well as cultured and primary cells. Here, we describe a method for simultaneous analysis of 33 different inflammatory cytokines and chemokines from mouse brain tissue using the Bio-Plex Pro Mouse Chemokine Panel 33-Plex.