Reciprocal gene expression of osteoclastogenesis inhibitory factor and osteoclast differentiation factor regulates osteoclast formation
Reciprocal gene expression of osteoclastogenesis inhibitory factor and osteoclast differentiation factor regulates osteoclast formation
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DOI：
10.1006/bbrc.1999.0524
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发表时间：
1999-04-21
影响因子：
3.1
通讯作者：
Sato, N
中科院分区：
文献类型：
--
作者：
Nagai, M;Sato, N
成骨细胞/基质细胞通过表达膜相关蛋白破骨细胞分化因子（ODF）支持破骨细胞祖细胞形成破骨细胞样细胞（OCL），而在未刺激状态下，细胞分泌大量的破骨细胞生成抑制因子（OCIF）。成骨细胞/基质细胞系中既有支持OCL形成的细胞系，也有非支持OCL形成的细胞系，其分化机制尚不清楚。本研究报告OCL形成支持细胞系ST 2显示ODF mRNA水平大大增加，而在1 α，25（OH）（2）-二羟基维生素D-3或前列腺素E-2存在下，其OCIF mRNA急剧减少。相比之下，缺乏OCL形成支持能力的MC 3 T3-E-1细胞并没有表现出OCIF mRNA对这些因素的反应减少，尽管ODF mRNA与ST 2细胞类似地增加。然而，失活的不分泌任何物质的MC 3 T3-E1细胞在与人早幼粒细胞HL 60共培养时支持OCL的形成。相反，当与1 α，25（OH）（2）维生素D-3处理的MC 3 T3-E1的条件培养基共培养时，ST 2细胞不支持HL 60细胞形成OCL。这些发现表明，成骨细胞/基质细胞中ODF和OCIF的相互基因表达对于支持OCL形成是必不可少的。(C)北京：科学出版社.
Osteoblasts/stromal cells support the formation of osteoclast-like cells (OCL) from osteoclast progenitor cells via expressing a membrane-associated protein, osteoclast differentiation factor (ODF), in the presence of osteotropic factors, whereas the cells secrete a substantial amount of osteoclastogenesis inhibitory factor (OCIF) in the unstimulated state. There are both OCL formation-supporting and the nonsupporting cell lines in osteoblasts/stromal cell lineages, The mechanism that divides osteoblasts/stromal cell lines into the two types is not known. The present study reports that OCL formation-supporting cell line ST2 showed a greatly increased level of ODF mRNA whereas their OCIF mRNA was drastically diminished in the presence of 1 alpha, 25(OH)(2)-dihydroxyvitamin D-3 or prostaglandin E-2. In contrast, MC3T3-E-1 cells lacking OCL formation-supporting ability did not show a decrease in OCIF mRNA in response to the factors, despite a similar increase in ODF mRNA as ST2 cells. However, inactivated MC3T3-E1 cells secreting nothing supported OCL formation in coculture with human promyelocytic cells, HL60, On the contrary, ST2 cells did not support OCL formation from HL60 cells when cocultured in medium conditioned by 1 alpha, 25(OH)(2) vitamin D-3-treated MC3T3-E1. These findings indicate that reciprocal gene expression of ODF and OCIF in osteoblasts/stromal cells is essential for supporting OCL formation. (C) 1999 Academic Press.