Expression of nitric oxide-containing structures in the rat carotid body
Expression of nitric oxide-containing structures in the rat carotid body
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DOI：
10.1016/j.acthis.2016.09.007
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发表时间：
2016-01-01
影响因子：
2.5
通讯作者：
Lazarov, Nikolai E.
中科院分区：
文献类型：
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作者：
Atanasova, Dimitrinka Y.;Dimitrov, Nikolay D.;Lazarov, Nikolai E.
颈动脉体（CB）是主要的外周动脉化学感受器器官，可引起代偿性反射反应以维持气体稳态。它由来自岩神经节 (PG) 神经元的感觉纤维、来自颈上神经节 (SCG) 的节后交感神经元的自主纤维和 CB 中内在神经节细胞的副交感血管舒缩纤维双重支配。使用烟酰胺腺嘌呤二核苷酸磷酸心肌黄酶 (NADPH-d) 组织化学、一氧化氮合酶 (NOS) 免疫组织化学和逆行法在大鼠 CB、PG 和 SCG 中检查一氧化氮 (NO) 的存在，一氧化氮 (NO) 是许多神经元和非神经元结构中假定的气态神经递质物质。 追踪。在 CB 中注射逆行示踪剂固蓝 (FB) 一周后，我们发现 PG 和 SCG 尾部的 Perikarya 子集被 FB 标记。组织化学和免疫组织化学显示，大多数大中型 PG 和 SCG 细胞呈 NADPH-d 阳性，并表现出强烈的 NOS 免疫染色。我们还观察到，许多穿透CB并包封血管球细胞和血管的曲张神经纤维具有NADPH-d反应性，并表达NOS、nNOS和eNOS的组成型亚型。此外，一些嵌入CB内或位于CB外周的自主微神经节细胞，而非血管球或支持细胞，呈nNOS免疫阳性，而CB微脉管系统表达eNOS。目前的结果表明，NO 是供应 CB 的自主神经末梢中的递质，并通过双重机制参与传出化学感受器抑制。 (C) 2016 爱思唯尔有限公司。版权所有。
The carotid body (CB) is a major peripheral arterial chemoreceptor organ that evokes compensatory reflex responses so as to maintain gas homeostasis. It is dually innervated by sensory fibers from petrosal ganglion (PG) neurons, and autonomic fibers from postganglionic sympathetic neurons of the superior cervical ganglion (SCG) and parasympathetic vasomotor fibers of intrinsic ganglion cells in the CB. The presence of nitric oxide (NO), a putative gaseous neurotransmitter substance in a number of neuronal and non-neuronal structures, was examined in the CB, PG and SCG of the rat using nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d) histochemistry, nitric oxide synthase (NOS) immunohistochemistry and retrograde tracing. One week after injecting the retrograde tracer Fast Blue (FB) in the CB, we found that a subset of perikarya in the caudal portions of the PG and SCG were FB-labeled. Histochemistry and immunohistochemistry revealed that the majority of large- and medium-sized PG and SCG cells were NADPH-d positive and displayed a strong NOS immunostaining. We also observed that many varicose nerve fibers penetrating the CB and enveloping the glomus cells and blood vessels were NADPH-d reactive and expressed the constitutive isoforms of NOS, nNOS and eNOS. In addition, some autonomic microganglion cells embedded within, or located at the periphery of the CB, and not glomus or sustentacular cells were nNOS-immunopositive while CB microvasculature expressed eNOS. The present results suggest that NO is a transmitter in the autonomic nerve endings supplying the CB and is involved in efferent chemoreceptor inhibition by a dual mechanism. (C) 2016 Elsevier GmbH. All rights reserved.