USE OF A MAMMALIAN INTERSPERSED REPETITIVE (MIR) ELEMENT IN THE CODING AND PROCESSING SEQUENCES OF MAMMALIAN GENES
USE OF A MAMMALIAN INTERSPERSED REPETITIVE (MIR) ELEMENT IN THE CODING AND PROCESSING SEQUENCES OF MAMMALIAN GENES
复制标题
DOI：
10.1093/nar/23.15.2837
复制
发表时间：
1995-08-11
影响因子：
14.9
通讯作者：
MORALES, JF
中科院分区：
文献类型：
--
作者：
MURNANE, JP;MORALES, JF
哺乳动物的散布重复(MIR)元件在1.3亿年前在哺乳动物中被扩增。MIR元件至少有260bp长，与哺乳动物基因组中的105个拷贝相似。我们分析了不同哺乳动物DNA中MIR元件的拷贝，以确定其与基因结构和功能的关系，试图确定MIR元件在哺乳动物基因组中的特定用途。我们发现，人类乙酰胆碱受体基因内的选择性剪接发生在MIR元件内，并导致部分MIR元件加入到该基因的编码序列中。此外，四种不同哺乳动物基因3‘端的多聚腺苷化信号(AATAAA)来源于MIR元件。MIR元件的这些用途表明，在哺乳动物基因组中发现的其他调控序列来自古老的转座元件，其中许多可能不再被识别。
The mammalian interspersed repetitive (MIR) element was amplified in mammals 130 million years ago. The MIR element is at least 260 bp in length and is found in similar to 105 copies in the mammalian genome. We analyzed copies of the MIR element in the DNA of various mammals to determine its relationship to the structure and function of genes, in an attempt to identify specific uses of the MIR element within the mammalian genome. We found that alternative splicing within the acetylcholine receptor gene in humans takes place within the MIR element and results in the incorporation of part of the MIR element into the coding sequence of this gene. Furthermore, the polyadenylation signal (AATAAA) at the 3' end of four different mammalian genes is derived from the MIR element. These uses of the MIR element suggest that other regulatory sequences found within the mammalian genome originated from ancient transposable elements, many of which may no longer be recognizable.