Progeny from sperm obtained after ectopic grafting of neonatal mouse testes
Progeny from sperm obtained after ectopic grafting of neonatal mouse testes
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DOI：
10.1095/biolreprod.102.014894
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发表时间：
2003-06-01
影响因子：
3.6
通讯作者：
Dobrinski, I
中科院分区：
文献类型：
--
作者：
Schlatt, S;Honaramooz, A;Dobrinski, I
睾丸组织异位移植是一种有前途的新方法，可用于保留睾丸功能。该技术最近已用于区分不同物种的新生儿睾丸，直至完全精子发生的水平。这种方法可以成功地应用于利用从新生儿捐赠者睾丸移植物中提取的精子来产生活体后代。在胞质内注射到小鼠卵母细胞中并将胚胎移植到代孕母亲体内后，精子能够支持正常发育并产生可育的雄性和雌性后代。移植组织还能够显着使去势受体的生殖激素水平正常化。这项技术为实验提供了新的途径。受体小鼠可以被视为睾丸组织的活体孵化器和培养系统，允许对睾丸发育和精子发生的多个方面进行实验操作。幼崽的成功产生表明该技术可用于研究睾丸表型并培育具有致死性出生后表型的突变或转基因小鼠品系。从生殖系离体产生精子的能力也为开发新策略以保留接受癌症治疗的男孩的生育能力铺平了道路。
Ectopic grafting of testicular tissue is a promising new approach that can be used to preserve testicular function. This technique has been used recently to differentiate the neonatal testes of different species, up to the level of complete spermatogenesis. This approach can be applied successfully to generate live progeny using sperm extracted from grafts originating from testes of newborn donors. The sperm are capable of supporting normal development and producing fertile male and female offspring after intracytoplasmic injection into mouse oocytes and embryo transfer into surrogate mothers. The grafted tissue was also capable of significantly normalizing reproductive hormone levels in the castrated recipients. This technique presents new avenues for experimentation. The recipient mouse can be regarded as a living incubator and a culture system of testicular tissue, allowing the experimental manipulation of several aspects of testis development and spermatogenesis. The successful generation of pups indicates that this technqiue can be used to study the testicular phenotype and to breed mutant or transgenic mouse strains with lethal postnatal phenotypes. The ability to generate sperm from the germ line ex vivo also paves the way for the development of new strategies for preserving fertility in boys undergoing cancer therapy.