Cultures of ligament fibroblasts in fibrin matrix gel
Cultures of ligament fibroblasts in fibrin matrix gel
复制标题
DOI：
10.1080/03008200390200210
复制
发表时间：
2003-03-01
影响因子：
2.9
通讯作者：
Nimni, ME
中科院分区：
文献类型：
--
作者：
Chun, J;Tuan, TL;Nimni, ME
在三维纤维蛋白基质凝胶(FMG)系统中分析了前交叉韧带(ACL)和内侧副韧带(MCL)成纤维细胞的细胞特性。在10%胎牛血清(FBS)中，MCL成纤维细胞的增殖速度明显快于ACL成纤维细胞。FMG收缩类似于软组织伤口收缩。在ACL和MCL成纤维细胞中，转化生长因子-β1(转化生长因子-β1)(5 ng/ml)引起FMG收缩的速度明显快于对照组(0.5%FBS)。与10%胎牛血清中的细胞不同，这种更快的FMG收缩速度是在不增加初始细胞数量的情况下实现的。在FMG中，培养第2~6天，MCL成纤维细胞的单位细胞胶原合成量明显高于ACL成纤维细胞。在体外观察到的ACL和MCL成纤维细胞的这些细胞特性的差异可能解释了这些韧带在体内愈合能力的差异。
The cellular properties of anterior cruciate ligament (ACL) and medial collateral ligament (MCL) fibroblasts have been analyzed in a three-dimensional fibrin matrix gel (FMG) system. The MCL fibroblasts proliferated significantly faster than ACL fibroblasts in 10% fetal bovine serum (FBS). FMG contraction resembles soft-tissue wound contraction. Transforming growth factor-beta1 (TGF-beta1) (5 ng/ml) caused a significantly faster rate of FMG contraction than control (0.5% FBS) in both ACL and MCL fibroblasts. Unlike the cells in 10% FBS, this faster rate of FMG contraction was achieved without increasing the initial cell number. In the FMG, the MCL fibroblasts demonstrated significantly higher collagen synthesis per cell than ACL fibroblasts between the days 2 and 6 of culture. These differences in cellular properties of the ACL and MCL fibroblasts that were observed in vitro may explain the differences in the healing potential of these ligaments in vivo.