Ultrasensitive and green electrochemical immunosensor for mycotoxin ochratoxin A based on phage displayed mimotope peptide
Ultrasensitive and green electrochemical immunosensor for mycotoxin ochratoxin A based on phage displayed mimotope peptide
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基于噬菌体展示模拟表位的霉菌毒素赭曲霉毒素A超灵敏绿色电化学免疫传感器
DOI：
10.1016/j.talanta.2018.10.081
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发表时间：
2019-03-01
期刊：
影响因子：
6.1
通讯作者：
He, Qing-hua
中科院分区：
文献类型：
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作者：
Hou, Si-lu;Ma, Zhen-e;He, Qing-hua
在这里，我们展示了一种开发赭曲霉毒素 A (OTA) 超灵敏绿色电化学免疫传感器的新方法。以噬菌体展示的OTA模拟表位肽作为传统竞争抗原的模拟物，以有毒霉菌毒素OTA为原料化学合成，在竞争性传感平台上进行。工作电极经过聚乙二醇（PEG）修饰，以有效固定抗体。在优化的测试条件下，所建立的免疫传感器的检测限（LOD）为2.04 fg/mL，线性范围为7.17-548.76 fg/mL。通过测试其他常见霉菌毒素的交叉反应性，对该建立的方法进行了特异性测量，结果表明基于模拟表位肽的免疫传感器与其他霉菌毒素的交叉反应性可以忽略不计。此外，噬菌体展示模拟表位肽免疫传感器的新概念可能为超灵敏检测食品中各种有毒小分子的通用方法提供潜在的应用。
Here, we demonstrated a new approach for development of an ultrasensitive and green electrochemical immunosensor for Ochratoxin A (OTA). Phage displayed mimotope peptide of OTA was used as mimics of conventional competing antigen, which is chemical synthesized with toxic mycotoxins OTA as raw material, in a competitive sensing platform. The working electrode was modified by polyethylene glycol (PEG) for the purpose of immobilizing antibody effectively. Under the optimized test condition, the limit of detection (LOD) of the established immunosensor was 2.04 fg/mL, and the linear range was 7.17-548.76 fg/mL. Specific measurement of this established method was conducted by testing cross-reactivity of other common mycotoxins, the result showed that mimotope peptide-based immunosensor has negligible cross-reactivity with other mycotoxins. Furthermore, the novel concept of phage displayed mimotope peptide-based immunosensor may provide a potential application in general method for the ultrasensitive detection of various toxic small molecules in food.