Distribution of core oligosaccharide types in lipopolysaccharides from Escherichia coli
Distribution of core oligosaccharide types in lipopolysaccharides from Escherichia coli
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DOI：
10.1128/iai.68.3.1116-1124.2000
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发表时间：
2000-03-01
影响因子：
3.1
通讯作者：
Whitfield, C
中科院分区：
文献类型：
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作者：
Amor, K;Heinrichs, DE;Whitfield, C
大肠杆菌的脂多糖有五种不同的核心寡糖（核心 OS）结构，指定为 K-12 和 R1 至 R4。这项工作的目的是确定这些核心操作系统类型在物种内的流行情况。 waa（核心 OS 生物合成）基因操纵子中的独特序列被用来开发基于 PCR 的系统，该系统有助于明确确定大肠杆菌分离株中的核心 OS 类型。该系统应用于大肠杆菌 ECOR 收集中的 72 个分离株，该分离株的汇编被认为广泛代表了该物种的遗传多样性。 50 个 (69.4%) 的 ECOR 分离株包含 R1 核心 OS，8 个 (11.1%) 是 R2 的代表，8 个 (11.1%) 是 R3，2 个 (2.8%) 是 R4，只有 4 个 (5.6%) 是 K-12。 R1 是 ECOR 集合中所有四个主要系统发育组（A、B1、B2 和 D）中发现的唯一核心操作系统类型。强毒肠外致病性大肠杆菌分离株往往与 B2 组密切相关，在较小程度上与 D 组分离株密切相关。 B2 和 D 组的所有 ECOR 代表都拥有 R1 核心操作系统。相比之下，共生大肠杆菌分离株与 A 组关系更密切，其中包含代表五个撕裂 OS 结构中每一个结构的分离株。 R3 是在来自人和牛的 38 种产毒性大肠杆菌 (VTEC) 分离株中发现的唯一核心 OS 类型，这些菌株属于常见的肠出血性大肠杆菌血清群 O157、O111 和 O26。尽管其他 VTEC 血清群的分离株搁置了更多核心 OS 多样性，但 R3 型（占所有 VTEC 分离株的 83.1%）仍然占主导地位。当对来自牛的非 VTEC 共生分离株进行分析时，发现大多数具有 R1 核心 OS 类型。
In the lipopolysaccharides of Escherichia coli there are five distinct core oligosaccharide (core OS) structures, designated K-12 and R1 to R4. The objective of this work was to determine the prevalences of these core OS types within the species. Unique sequences in the waa (core OS biosynthesis) gene operon were used to develop a PCR-based system that facilitated unequivocal determination of the core OS types in isolates of E. coli, This system was applied to the 72 isolates in the E. coli ECOR collection, a compilation of isolates that is considered to be broadly representative of the genetic diversity of the species. Fifty (69.4%) of the ECOR isolates contained the R1 core OS, 8 (11.1%) were representatives of R2, 8 (11.1%) were R3, 2 (2.8%) were R4, and only 4 (5.6%) were K-12. R1 is the only core OS type found in all four major phylogenetic groups (A, B1, B2, and D) in the ECOR collection. Virulent extraintestinal pathogenic E. coli isolates tend to be closely related to group B2 and, to a lesser extent, group D isolates. All of the ECOR representatives from the B2 and D groups had the R1 core OS. In contrast, commensal E. coli isolates are more closely related to group A, which contains isolates representing each of the five tore OS structures. R3 was the only core OS type found in 38 verotoxigenic E. coli (VTEC) isolates from humans and cattle belonging to the common enterohemorrhagic E. coli serogroups O157, O111, and O26, Although isolates from other VTEC serogroups shelved more core OS diversity, the R3 type (83.1% of all VTEC isolates) was still predominant. When non-VTEC commensal isolates from cattle were analyzed, it was found that most possessed the R1 core OS type.