MASS ISOLATION OF CALF THYMUS CENTROSOMES - IDENTIFICATION OF A SPECIFIC CONFIGURATION
MASS ISOLATION OF CALF THYMUS CENTROSOMES - IDENTIFICATION OF A SPECIFIC CONFIGURATION
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DOI：
10.1083/jcb.109.6.2869
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发表时间：
1989-12-01
影响因子：
7.8
通讯作者：
BORNENS, M
中科院分区：
文献类型：
--
作者：
KOMESLI, S;TOURNIER, F;BORNENS, M
从小牛胸腺细胞中心体分离使用一个简单的制备程序，提供大量的自由细胞器。一项与人类培养淋巴母细胞分离的中心体的比较研究发现，这两种分离的中心体在结构和从纯化的微管蛋白中形成微管的能力方面存在重要差异。讨论了中心体结构取决于细胞生长状态的可能性。
Centrosomes from calf thymocytes were isolated using a simple preparative procedure that provides large yields of free organelles. A comparative study with centrosomes isolated from human cultured lymphoblasts has led to the discovery of important differences in the structure of the two isolates and in their capacity to nucleate microtubules from purified tubulin. The possibility that the centrosomal structure depends upon the growth state of cells is discussed.