Seeding-Induced Self-assembling Protein Nanowires Dramatically Increase the Sensitivity of Immunoassays
Seeding-Induced Self-assembling Protein Nanowires Dramatically Increase the Sensitivity of Immunoassays
复制标题
接种诱导的自组装蛋白质纳米线显着提高了免疫测定的灵敏度
DOI：
10.1021/nl9003464
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发表时间：
2009-06-01
期刊：
影响因子：
10.8
通讯作者：
Zhang, Xian-En
中科院分区：
文献类型：
--
作者：
Men, Dong;Guo, Yong-Chao;Zhang, Xian-En
为了建立一个超灵敏和易于操作的免疫分析，双功能蛋白质纳米线产生的接种诱导自组装的酵母淀粉样蛋白Sup 35 p的基因融合蛋白G和酶（甲基-β-羟乙基淀粉水解酶，MPH），分别。蛋白质纳米线具有较高的酶分子与蛋白质G的比率，当蛋白质G与抗体靶标结合时，允许酶信号的急剧增加。结果表明，当应用于鼠疫耶尔森氏菌F1抗原的检测时，灵敏度提高了100倍。
Aiming to build a supersensitive and easily operable immunoassay, bifunctional protein nanowires were generated by seeding-induced self-assembling of the yeast amyloid protein Sup35p that genetically fused with protein G and an enzyme (methyl-parathion hydrolase, MPH), respectively. The protein nanowires possessed a high ratio of enzyme molecules to protein G, allowing a dramatic increase of the enzymatic signal when protein G was bound to an antibody target. As a result, a 100-fold enhancement of the sensitivity was obtained when applied in the detection of the Yersinia, pestis F1 antigen.