Muscle atrophy and fatty infiltration after an acute rotator cuff repair in a sheep model
Muscle atrophy and fatty infiltration after an acute rotator cuff repair in a sheep model
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DOI：
10.11138/mltj/2015.5.2.106
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发表时间：
2015-04-01
影响因子：
--
通讯作者：
Feeley, Brian T.
中科院分区：
文献类型：
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作者：
Luan, Tammy;Liu, Xuhui;Feeley, Brian T.
简介：肩袖撕裂（RCTs）是骨科患者最常见的肌腱损伤。肌肉萎缩和肌肉脂肪浸润是决定肩袖手术结果的关键因素。虽然在人类中的研究较少，但在动物模型中也观察到了肩袖肌肉纤维化，并且也可能影响结果。本研究的目的是确定即使在绵羊模型中进行急性修复的情况下，肩袖是否会发生肌肉变化。我们假设，脂肪浸润和纤维化将存在，即使在急性修复后6个月，初步surgery.Methods：12只成年雌性绵羊进行了急性肩袖撕裂和立即修复右肩。左肩作为对照，没有撕裂或修复。急性肩袖修复后6个月，收集绵羊肌肉，通过组织学分析、蛋白质印迹和逆转录聚合酶链反应（RT-PCR）研究肌萎缩、脂肪浸润和纤维化。肌腱修复组的肩袖肌肉中脂肪细胞、肌肉脂肪浸润和胶原沉积显著增加，与对照组相比，结论：即使肌腱在断裂后立即修复，肩袖肌肉也会发生包括脂肪浸润和纤维化在内的降解变化。证据等级：基础科学研究。
Introduction: rotator cuff tears (RCTs) are the most common tendon injury seen in orthopedic patients. Muscle atrophy and fatty infiltration of the muscle are crucial factors that dictate the outcome following rotator cuff surgery. Though less studied in humans, rotator cuff muscle fibrosis has been seen in animal models as well and may influence outcomes as well. The purpose of this study was to determine if the rotator cuff would develop muscle changes even in the setting of an acute repair in a sheep model. We hypothesized that fatty infiltration and fibrosis would be present even after an acute repair six months after initial surgery.Methods: twelve female adult sheep underwent an acute rotator cuff tear and immediate repair on the right shoulder. The left shoulder served as a control and did not undergo a tear or a repair. Six months following acute rotator cuff repairs, sheep muscles were harvested to study atrophy, fatty infiltration, and fibrosis by histological analysis, western blotting, and reverse transcription polymerase chain reaction (RT-PCR).Results: the repair group demonstrated an increase expression of muscle atrophy, fatty infiltration, and fibrosis related genes. Significantly increased adipocytes, muscle fatty infiltration, and collagen deposition was observed in rotator cuff muscles in the tendon repair group compared to the control group.Conclusions: rotator cuff muscle undergoes degradation changes including fatty infiltration and fibrosis even after the tendons are repair immediately after rupture.Level of Evidence: Basic Science Study.