Analysis of the transcriptome data in Litopenaeus vannamei reveals the immune basis and predicts the hub regulation-genes in response to high-pH stress
Analysis of the transcriptome data in Litopenaeus vannamei reveals the immune basis and predicts the hub regulation-genes in response to high-pH stress
复制标题
对凡纳滨对虾转录组数据的分析揭示了免疫基础并预测了响应高 pH 胁迫的中枢调节基因
DOI：
10.1371/journal.pone.0207771
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发表时间：
2018-12-05
期刊：
影响因子：
3.7
通讯作者：
Hu, Chaoqun
中科院分区：
文献类型：
--
作者：
Huang, Wen;Li, Hongmei;Hu, Chaoqun
土壤盐碱化侵蚀农田，严重威胁人类生存，盐碱地作为耕地资源的再利用日益突出。凡纳滨对虾（Litopenaeus vannamei）是盐碱区开发利用的重要养殖水生物种。然而，对该物种在高ph胁迫下的适应机制知之甚少。本研究采用转录组学方法，对高pH胁迫（pH 9.3±0.1）条件下凡纳虾鳃组织进行了转录组学分析。经分析，在cAMP信号通路（KO04024）中检测到环核苷酸门控通道- ca2 + (CNGC-Ca2+)和补丁1 （Ptc1）是主要的注释成分，表明CNGC-Ca2+和Ptc1可能分别是高ph胁迫信号转导和维持的候选成分。免疫球蛋白超家族（IgSF）、热休克蛋白（HSP）、谷胱甘肽s-转移酶（GST）、酚氧化酶原/酚氧化酶（proPO/PO）、超氧化物歧化酶（SOD）、抗脂多糖因子（ALF）和脂蛋白是高ph胁迫应答的主要转录免疫因子。为了进一步检测枢纽调控基因，构建了蛋白-蛋白相互作用（PPI）网络；“聚合酶（RNA） II （DNA导向）多肽A”（POLR2A）、“组蛋白乙酰转移酶p300”（EP300）和“热休克70kDa蛋白8”（HSPA8）基因/蛋白被认为是响应急性高ph胁迫的前3个枢纽调控基因；“热休克70kDa蛋白4”（HSPA4）、“FBJ小鼠骨肉瘤病毒癌基因同源物”（FOS）和“核孔蛋白54kDa”（NUP54）基因/蛋白被认为是参与适应耐力性高ph应激的前三大枢纽调控基因；发现“EP300-HSPA8”和“HSPA4-NUP54”蛋白相互作用是高ph胁迫下最重要的生物相互作用；HSP70家族基因可能在南美扁豆适应高ph胁迫环境中发挥重要作用。这些发现首次揭示了vannamei在高ph环境下的分子和免疫基础，PPI网络的构建有助于揭示虾类非生物胁迫响应的枢纽调控基因，并为进一步的研究奠定基础。
Soil salinization erodes the farmlands and poses a serious threat to human life, reuse of the saline-alkali lands as cultivated resources becomes increasingly prominent. Pacific white shrimp (Litopenaeus vannamei) is an important farmed aquatic species for the development and utilization of the saline-alkali areas. However, little is known about the adaptation mechanism of this species in terms of high-pH stress. In the present study, a transcriptome analysis on the gill tissues of L. vannamei in response to high-pH stress (pH 9.3 ± 0.1) was conducted. After analyzing, the cyclic nucleotide gated channel-Ca2+ (CNGC-Ca2+) and patched 1 (Ptc1) were detected as the majority annotated components in the cAMP signaling pathway (KO04024), indicating that the CNGC-Ca2+ and Ptc1 might be the candidate components for transducing and maintaining the high-pH stress signals, respectively. The immunoglobulin superfamily (IgSF), heat shock protein (HSP), glutathione s-transferase (GST), prophenoloxidase/phenoloxidase (proPO/PO), superoxide dismutase (SOD), anti-lipopolysaccharide factor (ALF) and lipoprotein were discovered as the major transcribed immune factors in response to high-pH stress. To further detect hub regulation-genes, protein-protein interaction (PPI) networks were constructed; the genes/proteins “Polymerase (RNA) II (DNA directed) polypeptide A” (POLR2A), “Histone acetyltransferase p300” (EP300) and “Heat shock 70kDa protein 8” (HSPA8) were suggested as the top three hub regulation-genes in response to acute high-pH stress; the genes/proteins “Heat shock 70kDa protein 4” (HSPA4), “FBJ murine osteosarcoma viral oncogene homolog” (FOS) and “Nucleoporin 54kDa” (NUP54) were proposed as the top three hub regulation-genes involved in adapting endurance high-pH stress; the protein-interactions of “EP300-HSPA8” and “HSPA4-NUP54” were detected as the most important biological interactions in response to the high-pH stress; and the HSP70 family genes might play essential roles in the adaptation of the high-pH stress environment in L. vannamei. These findings provide the first insight into the molecular and immune basis of L. vannamei in terms of high-pH environments, and the construction of a PPI network might improve our understanding in revealing the hub regulation-genes in response to abiotic stress in shrimp species and might be beneficial for further studies.