Increased memory differentiation is associated with decreased polyfunctionality for HIV but not for cytomegalovirus-specific CD8+ T cells.
Increased memory differentiation is associated with decreased polyfunctionality for HIV but not for cytomegalovirus-specific CD8+ T cells.
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DOI：
10.4049/jimmunol.1201488
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发表时间：
2012-10-15
期刊：
影响因子：
--
通讯作者：
CAPRISA 002 Study Team
中科院分区：
文献类型：
--
作者：
Riou C;Treurnicht F;Abrahams MR;Mlisana K;Liu MK;Goonetilleke N;Koup R;Roederer M;Abdool Karim S;de Bruyn G;Williamson C;Gray CM;Burgers WA;CAPRISA 002 Study Team
响应疫苗接种或自然感染而产生的多功能 CD8+ T 细胞与保护性免疫力的提高有关。然而，尚不清楚多功能性的维持是否与特定的细胞表型特征有关。为了确定细胞因子表达谱是否与记忆分化阶段相关，我们分析了 20 名首次接受 ART 的 HIV-1 感染者在感染后约 34 周时不同记忆亚群中 HIV 特异性 CD8+ T 细胞的多功能程度。将这些特征与未感染 HIV 的对照组和长期感染 HIV 的个体的 CMV 特异性 CD8+ T 细胞反应进行比较。我们的结果表明，HIV 特异性 CD8+ T 细胞的多功能能力因其记忆表型而异。与终末分化 (CD45RO−CD27−) HIV 特异性 CD8+ T 细胞相比，早期分化细胞 (CD45RO+CD27+) 表现出较高比例的三或四种功能阳性细胞 (p<0.001)，以及较低比例的单功能细胞 (p<0.001)。大多数终末分化的 HIV 特异性 CD8+ T 细胞是单功能的（中位数 69% [IQR: 57–83]），主要产生 CD107a 或 MIP1β。此外，HIV特异性单功能CD8+ T细胞的比例与终末分化的HIV特异性CD8+ T细胞的比例呈正相关（p=0.019，r=0.54）。相比之下，CMV 特异性 CD8+ T 细胞多功能能力在所有记忆亚群中都相似，终末分化和早期分化细胞具有相当的多功能性。总体而言，这些数据表明，HIV 特异性 CD8+ T 细胞的多功能能力受到记忆分化阶段的影响，而 CMV 特异性反应则不然。
The generation of polyfunctional CD8+ T cells, in response to vaccination or natural infection, has been associated with improved protective immunity. However, it is unclear whether the maintenance of polyfunctionality is related to particular cellular phenotypic characteristics. To determine whether the cytokine expression profile is linked to the memory differentiation stage, we analyzed the degree of polyfunctionality of HIV-specific CD8+ T cells within different memory subpopulations in 20 ART-naïve HIV-1 infected individuals at approximately 34 weeks post infection. These profiles were compared with CMV-specific CD8+ T cell responses in HIV-uninfected controls and in individuals chronically infected with HIV. Our results showed that the polyfunctional abilities of HIV-specific CD8+ T cells differed according to their memory phenotype. Early-differentiated cells (CD45RO+CD27+) exhibited a higher proportion of cells positive for three or four functions (p<0.001), and a lower proportion of mono-functional cells (p<0.001) compared to terminally-differentiated (CD45RO−CD27−) HIV-specific CD8+ T cells. The majority of terminally-differentiated HIV-specific CD8+ T cells were mono-functional (median 69% [IQR: 57–83]), producing predominantly CD107a or MIP1β. Moreover, proportions of HIV-specific mono-functional CD8+ T cells positively associated with proportions of terminally-differentiated HIV-specific CD8+ T cells (p=0.019, r=0.54). In contrast, CMV-specific CD8+ T cell polyfunctional capacities were similar across all memory subpopulations, with terminally- and early-differentiated cells endowed with comparable polyfunctionality. Overall, these data show that the polyfunctional abilities of HIV-specific CD8+ T cells are influenced by the stage of memory differentiation, which is not the case for CMV-specific responses.
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DOI：
10.1084/jem.20070784
发表时间：
2007-10-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Almeida JR;Price DA;Papagno L;Arkoub ZA;Sauce D;Bornstein E;Asher TE;Samri A;Schnuriger A;Theodorou I;Costagliola D;Rouzioux C;Agut H;Marcelin AG;Douek D;Autran B;Appay V
通讯作者：
Appay V
影响因子：
6.7
作者：
Chopera DR;Woodman Z;Mlisana K;Mlotshwa M;Martin DP;Seoighe C;Treurnicht F;de Rosa DA;Hide W;Karim SA;Gray CM;Williamson C;CAPRISA 002 Study Team
通讯作者：
CAPRISA 002 Study Team
影响因子：
20.3
作者：
Hersperger, Adam R.;Martin, Jeffrey N.;Betts, Michael R.
通讯作者：
Betts, Michael R.
影响因子：
5.4
作者：
Jagannathan, Prasanna;Osborne, Christine M.;Migueles, Stephen A.
通讯作者：
Migueles, Stephen A.
影响因子：
64.8
作者：
Champagne, P;Ogg, GS;Pantaleo, G
通讯作者：
Pantaleo, G