Using a metal oxide sensor (MOS)-based electronic nose for discrimination of bacteria based on individual colonies in suspension
Using a metal oxide sensor (MOS)-based electronic nose for discrimination of bacteria based on individual colonies in suspension
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DOI：
10.1016/j.snb.2010.09.062
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发表时间：
2011-02-20
影响因子：
8.4
通讯作者：
Lin, Min
中科院分区：
文献类型：
--
作者：
Green, Geoffrey C.;Chan, Adrian D. C.;Lin, Min
及时准确地测定食源性细菌病原体的存在和性质对于食品安全至关重要。在本文中，我们研究了电子鼻（基于金属氧化物传感器阵列）是否能够区分大肠杆菌和李斯特菌。与该领域的其他研究不同，样品由从琼脂平板转移的单个菌落组成，然后悬浮在磷酸盐缓冲盐水中。从传感器响应曲线中提取的特征捕获静态（稳态）和动态（斜率）信息。使用线性分类器与监督降维相结合，使用不相关线性判别分析 (ULDA)，产生了 92.4% 的分类准确率。所提出的方法有可能减少识别细菌病原体所需的总时间。这种类型的样品在检查过程中相对较早即可获得，因此基于单个菌落的气味特征的区分有可能通过消除或减少后续的培养阶段和生化测试来减少时间和成本。本文提出的结果表明，有必要在这一领域进行进一步的研究，特别是针对更广泛的细菌种类。 (c) 2010 Elsevier B.V. 保留所有权利。
Timely and accurate determination of the presence and nature of foodborne bacterial pathogens is of utmost importance in food safety. In this paper, we investigated whether an electronic nose (based on a metal-oxide sensor array) could distinguish between E. coli and Listeria. Unlike other studies in this area, samples consisted of individual colonies transferred from agar plates, then suspended in phosphate buffered saline. Features extracted from the sensor response curves capture both static (steady state) and dynamic (slope) information. The use of a linear classifier in association with supervised dimensionality reduction, using uncorrelated linear discriminant analysis (ULDA), yielded classification accuracies of 92.4%. The proposed method has the potential to reduce the overall time required to identify bacterial pathogens. This type of sample is available relatively early in the inspection process, so discrimination based on the odour signature of single colonies has the potential to reduce time and cost by eliminating or reducing subsequent culturing stages and biochemical testing. The results presented herein suggest that further research in this area is warranted, particularly with a wider variety of bacterial species. (c) 2010 Elsevier B.V. All rights reserved.