Axon Branch-Specific Semaphorin-1a Signaling in Drosophila Mushroom Body Development
Axon Branch-Specific Semaphorin-1a Signaling in Drosophila Mushroom Body Development
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DOI：
10.3389/fncel.2016.00210
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发表时间：
2016-09
影响因子：
5.3
通讯作者：
L. Zwarts;T. Goossens;J. Clements;Y. Kang;P. Callaerts
中科院分区：
文献类型：
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作者：
L. Zwarts;T. Goossens;J. Clements;Y. Kang;P. Callaerts
果蝇大脑中蘑菇体（MB）神经元的正确布线涉及不同轴突叶的适当定位，以及从单个轴突发育的姐妹分支。这种定位需要整合不同细胞类型提供的各种指导线索，这有助于轴突找到它们在神经元内的最终位置。脑信号蛋白在神经元发育和轴突导向中具有保守的作用。我们更密切地调查了Sema-1a在MB开发中的作用。我们发现，Sema-1a表达的MBs以及周围的结构，包括胶质细胞短暂的大脑半球间纤维环，在整个发展。通过功能丧失和获得实验，我们发现MB轴突显示叶和姐妹分支特异性Sema-1a信号传导，其控制轴突生长和指导的不同方面。此外，我们表明，这些影响是由MB的内在和外在的Sema-1a信号通路，涉及PlexA和PlexB的整合调制。最后，我们还显示了神经元-胶质细胞相互作用在Sema-1a依赖性β叶生长中的作用。
Correct wiring of the mushroom body (MB) neuropil in the Drosophila brain involves appropriate positioning of different axonal lobes, as well as the sister branches that develop from individual axons. This positioning requires the integration of various guidance cues provided by different cell types, which help the axons find their final positions within the neuropil. Semaphorins are well-known for their conserved roles in neuronal development and axon guidance. We investigated the role of Sema-1a in MB development more closely. We show that Sema-1a is expressed in the MBs as well as surrounding structures, including the glial transient interhemispheric fibrous ring, throughout development. By loss- and gain-of-function experiments, we show that the MB axons display lobe and sister branch-specific Sema-1a signaling, which controls different aspects of axon outgrowth and guidance. Furthermore, we demonstrate that these effects are modulated by the integration of MB intrinsic and extrinsic Sema-1a signaling pathways involving PlexA and PlexB. Finally, we also show a role for neuronal- glial interaction in Sema-1a dependent β-lobe outgrowth.