Benchmarking of fluorescence cameras through the use of a composite phantom
Benchmarking of fluorescence cameras through the use of a composite phantom
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DOI：
10.1117/1.jbo.221.016009
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发表时间：
2017-01-01
影响因子：
3.5
通讯作者：
Ntziachristos, Vasilis
中科院分区：
文献类型：
--
作者：
Gorpas, Dimitris;Koch, Maximilian;Ntziachristos, Vasilis
荧光分子成像（FMI）已经显示出在手术或诊断内窥镜检查中检测和描绘癌症的潜力。成像系统的最新进展使得即使在视频速率模式下也能灵敏地检测荧光剂。然而，荧光成像缺乏标准化对FMI的临床应用提出了挑战，因为即使使用相同的荧光剂，使用不同的系统也可能导致给定研究的不同结果。在这项工作中，我们研究了复合荧光模体的使用，作为FMI标准，为不同成像系统的性能验证和标准化提供了一种综合方法。为了排除用户交互，使用适当构建的模板，从获得的外照彩色和荧光图像中自动提取所有幻像特征。这些特征然后被用来描述性能和比较不同的相机彼此。所提出的方法可以作为FMI系统校准和基准测试的框架，以促进其临床转化。(C) 2017年中国光学仪器工程师学会（SPIE）
Fluorescence molecular imaging (FMI) has shown potential to detect and delineate cancer during surgery or diagnostic endoscopy. Recent progress on imaging systems has allowed sensitive detection of fluorescent agents even in video rate mode. However, lack of standardization in fluorescence imaging challenges the clinical application of FMI, since the use of different systems may lead to different results from a given study, even when using the same fluorescent agent. In this work, we investigate the use of a composite fluorescence phantom, employed as an FMI standard, to offer a comprehensive method for validation and standardization of the performance of different imaging systems. To exclude user interaction, all phantom features are automatically extracted from the acquired epi-illumination color and fluorescence images, using appropriately constructed templates. These features are then employed to characterize the performance and compare different cameras to each other. The proposed method could serve as a framework toward the calibration and benchmarking of FMI systems, to facilitate their clinical translation. (C) 2017 Society of Photo-Optical Instrumentation Engineers (SPIE)