Spontaneous [Ca2+]i oscillations in G1/S phase-synchronized cells
Spontaneous [Ca2+]i oscillations in G1/S phase-synchronized cells
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DOI：
10.1093/jmicro/dfp023
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发表时间：
2009-10-01
期刊：
影响因子：
--
通讯作者：
Satoh, Yoh-ichi
中科院分区：
文献类型：
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作者：
Russa, Afadhali Denis;Maesawa, Chihaya;Satoh, Yoh-ichi
Ca 2+信号转导控制着细胞的多种功能，如分裂、受精、凋亡和坏死。具体来说，钙信号被认为在驱动细胞通过细胞分裂周期的不同阶段中起着至关重要的作用。然而，在大多数细胞中，这一事实远未确立。很少有研究从不同的角度研究这个问题：细胞是否表现出一些特征性的细胞周期依赖性细胞内钙信号模式。这种方法是有效的识别Ca 2+信号和细胞周期之间的因果关系。通过细胞周期同步化、流式细胞仪和共聚焦扫描显微镜观察细胞内钙离子浓度（[Ca 2 +]（i））成像，本研究表明，G1/S相变的独特特征是持续长达40分钟的自发[Ca 2 +]（i）振荡。这些振荡源自[Ca 2 +]（i）信号，其伴随着DNA复制，细胞为下一个分裂周期做准备。这些时间信号进一步证实了Ca 2+在细胞周期中的重要性。
Ca2+ signaling controls a wide range of cellular functions such as division, fertilization, apoptosis and necrosis. Specifically, calcium signaling is thought to play a crucial role in driving cells through the different stages of the cell-division cycle. In most cells, however, this fact is far from being established. Few studies have examined this question from a different perspective: whether cells exhibit some characteristic cell cycle-dependent intracellular calcium-signaling patterns. This approach is effective in discerning the causal relationship between Ca2+ signaling and the cell cycle. Through synchronization of the cell cycle, flow cytometry and confocal scanning microscopic intracellular calcium ion concentration ([Ca2+](i)) imaging, the present study shows that the G1/S phase transition is uniquely characterized by spontaneous [Ca2+](i) oscillations that last for up to 40 min. Most likely, these oscillations emanate from the [Ca2+](i) signaling that accompanies DNA replication as the cell prepares for the next division cycle. These temporal signals further affirm the significance of Ca2+ in the cell cycle.