Horseradish peroxidase-catalyzed polymerization of L-DOPA for mono-/bi-enzyme immobilization and amperometric biosensing of H2O2 and uric acid
Horseradish peroxidase-catalyzed polymerization of L-DOPA for mono-/bi-enzyme immobilization and amperometric biosensing of H2O2 and uric acid
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辣根过氧化物酶催化的 L-DOPA 聚合用于单酶/双酶固定以及 H2O2 和尿酸的电流生物传感
DOI：
10.1016/j.talanta.2015.11.047
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发表时间：
2016-03-01
期刊：
影响因子：
6.1
通讯作者：
Meng, Wenhua
中科院分区：
文献类型：
--
作者：
Dai, Mengzhen;Huang, Ting;Meng, Wenhua
在H2 O2（和尿酸酶（UOx））存在下，辣根过氧化物酶（HRP）催化L-DOPA（vs.多巴胺）的聚合反应，用于单酶/双酶催化的H2 O2和尿酸（UA）的电流生物传感。在含有HRP和L-DOPA（或加UOx）的磷酸盐缓冲溶液中加入H_2O_2后制备相应的聚合物生物复合材料（PBCs）。通过简单地将一些PBMC浇铸在镀有Au的Au（Au-plate/Au）电极上，然后用壳聚糖（CS）膜为每个涂层来制备单/双酶安培生物传感器。紫外-可见分光光度法，扫描电子显微镜，循环伏安法和电化学阻抗谱用于膜表征和/或过程监测。紫外-可见分光光度法证实酶催化固定化的HRP保持了其生物活性。在优化的条件下，单酶CS/HRP-聚（L-DOPA）（PD）/Au-板/Au电极在-0.1 V电位下对0.001 - 1.25 mM的H2 O2浓度有线性响应，灵敏度为700 μ A mM（-1）cm（-2），检测限（LOD）为0.1 μ M，双酶CS/UOx-HRP-PD/Au-plate/Au电极在0.1 V下对UA浓度在0.001 - 0.4 mM范围内呈线性响应，灵敏度为349 μ A mM（-1）cm（-2），检测限为0.1 μ M。基于生物合成的PD的单/双酶生物传感器比许多报道的类似物和基于类似生物合成的聚多巴胺的生物传感器表现更好。(C)2015 Elsevier B. V.版权所有。
Horseradish peroxidase (HRP)-catalyzed polymerization of L-DOPA (vs. dopamine) in the presence of H2O2 (and uricase (UOx)) was exploited to immobilize mono-/bi-enzymes for hydroquinone-mediated amperometric biosensing of H2O2 and uric acid (UA). The relevant polymeric biocomposites (PBCs) were prepared in phosphate buffer solution containing HRP and L-DOPA (or plus UOx) after adding H2O2. The mono-/bi-enzyme amperometric biosensors were prepared simply by casting some of the PBCs on Au plated Au (Au-plate/Au) electrodes, followed by coating with an outer-layer chitosan (CS) film for each. UV-vis spectrophotometry, scanning electron microscopy, cyclic voltammetry and electrochemical impedance spectroscopy were used for film characterization and/or process monitoring. The HRP immobilized by enzyme catalysis well preserved its bioactivity, as confirmed by UV-vis spectrophotometry. Under optimized conditions, the monoenzyme CS/HRP-poly(L-DOPA) (PD)/Au-plate/Au electrode potentiostated at -0.1 V responded linearly to H2O2 concentration from 0.001 to 1.25 mM with a sensitivity of 700 mu A mM(-1) cm(-2) and a limit of detection (LOD) of 0.1 mu M, and the bienzyme CS/UOx-HRP-PD/Au-plate/Au electrode at 0.1 V responded linearly to UA concentration from 0.001 to 0.4 mM with a sensitivity of 349 mu A mM(-1) cm(-2) and a LOD of 0.1 mu M. The mono-/bi-enzyme biosensors based on biosynthesized PD performed better than many reported analogues and those based on similarly biosynthesized polydopamine. (C) 2015 Elsevier B.V. All rights reserved.