Ischemic preconditioning improves preservation with crystalloid cardioplegia.
Ischemic preconditioning improves preservation with crystalloid cardioplegia.
复制标题
缺血预处理可改善晶体停跳液的保存。
DOI：
10.1016/0003-4975(94)91940-2
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Adrian Tristan
中科院分区：
文献类型：
--
作者：
R. Illes;John K. Wright;K. Inners;Chunjie Yang;Adrian Tristan
缺血预处理尚未在临床相关的低温多剂量心脏停搏模型中进行研究。用离体兔心在Langendorff装置上灌流，在15 °C缺血2.5小时期间，研究缺血预处理作为晶体心脏停搏液的辅助。在获得基线功能数据后，通过1分钟或5分钟的常温缺血诱导缺血预处理，然后在停搏期前再灌注5分钟。对照心脏未接受缺血预处理。对照组心脏的峰值压力和舒张功能均降低，舒张压-容积关系的斜率分别为107 ± 2至68 ± 7 mm Hg（p< 0.005）和0.99 ± 0.2至2.95 ± 0.44 mm Hg/0.1 mL（p< 0.005）。暴露于1或5分钟常温缺血的心脏显示舒张压-容积关系的斜率没有显著变化。暴露于常温缺血1或5分钟的心脏也具有峰值发展压力的显著降低，分别从107 ± 6至92 ± 2 mm Hg和从102 ± 3至85 ± 4 mm Hg（p< 0.05）。然而，与对照组相比，缺血预处理显著改善了缺血后峰发展压力（p< 0.05）。肌酸激酶洗脱仅在对照心脏中显著更高。高能磷酸盐水平、乳酸盐水平、湿重百分比和组织磷酸肌酸水平在各组之间没有显著差异。我们的研究结果表明，缺血预处理可能是有用的情况下，预期延长的交叉钳制期，如果这种效果是通过未来的调查验证。
Ischemic preconditioning has not been investigated in a clinically relevant model of hypothermic multidose cardioplegia arrest. Using isolated rabbit hearts perfused on a Langendorff apparatus, ischemic preconditioning was investigated as an adjunct to crystalloid cardioplegia during a 2.5-hour ischemic period at 15 °C. After baseline functional data were obtained, ischemic preconditioning was induced with either 1 minute or 5 minutes of normothermic ischemia, followed by 5 minutes of reperfusion before the arrest period. Control hearts underwent no ischemic preconditioning. The control hearts exhibited a decrement in both the peak developed pressure and diastolic function, as measured by the slope of the diastolic pressure-volume relationship, of from 107 ± 2 to 68 ± 7 mm Hg (p< 0.005) and from 0.99 ± 0.2 to 2.95 ± 0.44 mm Hg/0.1 mL (p< 0.005), respectively. Hearts exposed to either 1 or 5 minutes of normothermic ischemia showed no significant change in the slope of the diastolic pressure-volume relationship. Hearts exposed to 1 or 5 minutes of normothermic ischemia also had a significant decrease in the peak developed pressure of from 107 ± 6 to 92 ± 2 mm Hg and from 102 ± 3 to 85 ± 4 mm Hg, respectively (p< 0.05). However, ischemic preconditioning brought about a significant improvement in the postischemic peak developed pressure, as opposed to that seen for the control hearts (p< 0.05). Creatine kinase washout was significantly higher in the control hearts only. High-energy phosphate levels, lactate levels, the percentage wet weight, and tissue creatine phosphate levels were not significantly different among the groups. Our findings suggest ischemic preconditioning may be useful in cases with an anticipated prolonged cross-clamp period, if this effect is validated by future investigations.
通过反复击晕对心脏进行预处理。
DOI：
--
发表时间：
1992
期刊：
Cellular and molecular biology (Noisy-le-Grand, France)
影响因子：
--
作者：
Das,DK;Prasad,MR;Lu,D;Jones,RM
通讯作者：
Jones,RM
影响因子：
20.1
作者：
MURRY, CE;RICHARD, VJ;JENNINGS, RB
通讯作者：
JENNINGS, RB