WATER DEFICIT AND ABSCISIC-ACID CAUSE DIFFERENTIAL INHIBITION OF SHOOT VERSUS ROOT-GROWTH IN SOYBEAN SEEDLINGS - ANALYSIS OF GROWTH, SUGAR ACCUMULATION, AND GENE-EXPRESSION
WATER DEFICIT AND ABSCISIC-ACID CAUSE DIFFERENTIAL INHIBITION OF SHOOT VERSUS ROOT-GROWTH IN SOYBEAN SEEDLINGS - ANALYSIS OF GROWTH, SUGAR ACCUMULATION, AND GENE-EXPRESSION
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DOI：
10.1104/pp.92.1.205
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发表时间：
1990-01-01
期刊：
影响因子：
7.4
通讯作者：
MULLET, JE
中科院分区：
文献类型：
--
作者：
CREELMAN, RA;MASON, HS;MULLET, JE
根经常继续伸长，而粪便生长受到抑制，在植物受到低水势。这种差异的响应水分亏缺的原因进行了调查。以黄化大豆（Glycine max [L.] Merr. cv威廉姆斯）幼苗，其生长受到低水势蛭石或外源阿坝的抑制。两种处理以相似的方式影响生长和干重。下胚轴生长的最大抑制发生时，内部阿坝水平（阿坝应用调制）达到内源性水平，发现在生长在缺水蛭石幼苗的伸长区。相反，根的生长受到影响，只有轻微的程度在低水势幼苗和大多数阿坝治疗（在一些，促进生长）。在每一个幼苗部分检查，转移到低水势蛭石幼苗阿坝水平增加约5至10倍，发现在良好的浇水幼苗。可溶性糖含量的变化，多核糖体状态，和多核糖体mRNA翻译产物中看到的低水势幼苗没有发生阿坝处理足以引起显着抑制下胚轴伸长。这些数据表明，无论是内源阿坝水平的变化，和不同的敏感性阿坝的下胚轴和根可以调节根/芽生长比。然而，外源阿坝并没有引起糖积累的变化，多核糖体状态，和mRNA的人口后，转移到低水势蛭石。
Roots often continue to elongate while stool growth is inhibited in plants subjected to low-water potentials. The cause of this differential response to water deficit was investigated. We examined hypocotyl and root growth, polysome status and mRNA population, and abscisic acid (ABA) content in etiolated soybean (Glycine max [L.] Merr. cv Williams) seedlings whose growth was inhibited by transfer to low-water potential vermiculite or exogenous ABA. Both treatments affected growth and dry weight in a similar fashion. Maximum inhibition of hypocotyl growth occurred when internal ABA levels (modulated by ABA application) reached the endogenous level found in the elongating zone of seedlings grown in water-deficient vermiculite. Conversely, root growth was affected to only a slight extent in low-water potential seedlings and by most ABA treatments (in some, growth was promoted). In every seedling section examined, transfer of seedlings into low-water potential vermiculite caused ABA levels to increase approximately 5- to 10-fold over that found in well-watered seedlings. Changes in soluble sugar content, polysome status, and polysome mRNA translation products seen in low-water potential seedlings did not occur with ABA treatments sufficient to cause significant inhibition of hypocotyl elongation. These data suggest that both variation in endogenous ABA levels, and differing sensitivity to ABA in hypocotyls and roots can modulate root/shoot growth ratios. However, exogenous ABA did not induce changes in sugar accumulation, polysome status, and mRNA populations seen after transfer into low-water potential vermiculite.