A novel substrate-inspired fluorescent probe to monitor native albumin in human plasma and living cells
A novel substrate-inspired fluorescent probe to monitor native albumin in human plasma and living cells
复制标题
一种新型底物激发荧光探针，用于监测人血浆和活细胞中的天然白蛋白
DOI：
10.1016/j.aca.2017.07.048
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发表时间：
2017-10-09
影响因子：
6.2
通讯作者：
Yang, Ling
中科院分区：
文献类型：
--
作者：
Ge, Guang-Bo;Feng, Lei;Yang, Ling
人类白蛋白（HA）在维持健康和对抗疾病方面发挥着至关重要的作用。准确测定血浆或非血浆样品中的天然透明质酸在基础研究和临床实践中都具有重要意义。本文设计并开发了一种新型比例双光子荧光探针（n -丁基-4-（4-苯基-苯甲酰氧基）1,8-萘酰亚胺，BPBN），基于其独特的伪酯酶特性，用于高选择性和高灵敏度地检测HA的酶活性。BPBN在生理条件下表现出优良的选择性、高灵敏度和良好的反应性。作为一种基于酶活性的探针，BPBN可以区分天然HA和变性HA，而目前使用的染料结合方法无法区分。该探针已成功应用于测定血浆样品中的天然透明质酸和肝细胞培养上清中的分泌透明质酸。BPBN还被用于活体肾细胞HA重吸收的双光子成像，结果表明该探针具有良好的细胞通透性，低细胞毒性和高成像分辨率。这些结果表明，BPBN可以可靠地用于复杂生物样品中天然HA的高选择性和高灵敏度检测，以及探索HA相关的生物过程和活细胞中天然HA的生理功能。(C) 2017 Elsevier B.V.版权所有
Human albumin (HA) displays crucial roles in maintaining health and fighting diseases. Accurate determination of native HA in plasma or non-plasma samples are of immense significance in both basic research and clinical practice. Herein, a novel ratiometric two-photon fluorescent probe (N-butyl-4-(4-phenyl-benzoyloxy) 1,8-naphthalimide, BPBN) has been designed and developed for highly selective and sensitive sensing the enzymatic activities of HA, on the basis of its unique pseudo-esterase feature. BPBN exhibits excellent selectivity, high sensitivity and good reactivity under physiological conditions. As an enzymatic activity-based probe, BPBN can distinguish between native HA and denatured HA, while the currently used dye-binding method cannot. The probe has been successfully applied to measure native HA in plasma samples and the secreted HA in the hepatocyte culture supernatant. BPBN has also been used for two-photon imaging of HA reabsorption in living renal cells, and the results demonstrate that this probe exhibits good cell permeability, low cytotoxicity and high imaging resolution. All these findings suggest that BPBN can be reliably used for the highly selective and sensitive detection of native HA in complex biological samples, as well as for exploring HA-associated biological processes and the physiological functions of native HA in living cells. (C) 2017 Elsevier B.V. All rights reserved.