Carbon monoxide-dependent chemolithotrophic growth of Clostridium thermoautotrophicum.
Carbon monoxide-dependent chemolithotrophic growth of Clostridium thermoautotrophicum.
复制标题
热自养梭菌的一氧化碳依赖性化能自养生长。
DOI：
10.1128/aem.53.8.1902-1906.1987
复制
发表时间：
1987
影响因子：
4.4
通讯作者：
Drake,HL
中科院分区：
文献类型：
--
作者：
Savage,MD;Wu,ZG;Daniel,SL;LundieJr,LL;Drake,HL
热自养梭菌（Clostridium thermoautotrophicum）是在共同依赖的化学营养条件下培养的。CO依赖的生长曲线和能量学表明，以CO为代价，补充CO2是有效生长的基础。总体化学计量学约为6.5CO----CH3CO2H + 3.5CO2 + 0.6细胞C + 0.5未回收C。初始CO/CO2比为2至4，可获得最佳的倍增时间和细胞产量。最大YCO值约为每消耗1 mol CO细胞干重2.5 g；YH2明显低于YCO。交叉转移生长实验和蛋白质谱显示CO和甲醇培养物之间基因表达差异。
The acetogen Clostridium thermoautotrophicum was cultivated under CO-dependent chemolithotrophic conditions. CO-dependent growth profiles and energetics indicated that supplemental CO2 was fundamental to efficient growth at the expense of CO. Overall product stoichiometry approximated 6.5CO----CH3CO2H + 3.5CO2 + 0.6 cell C + 0.5 unrecovered C. Initial CO/CO2 ratios of 2 to 4 yielded optimal doubling times and cell yields. Maximal YCO values approximated 2.5 g of cell dry weight per mol of CO consumed; YH2 was considerably lower than YCO. Cross-transfer growth experiments and protein profiles indicated differential expression of genes between CO and methanol cultures.