Lower expression of genes near microRNA in C-elegans germline
Lower expression of genes near microRNA in C-elegans germline
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DOI：
10.1186/1471-2105-7-112
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发表时间：
2006-03-06
影响因子：
3
通讯作者：
Kohane, IS
中科院分区：
文献类型：
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作者：
Inaoka, H;Fukuoka, Y;Kohane, IS
背景：MicroRNA（miRNAs）是近年来发现的一类非蛋白质编码的短RNA分子。miRNA越来越多地参与组织特异性转录控制，特别是在发育中。由于有越来越多的证据表明，转录控制的局部组件，我们调查是否有一个距离依赖性的影响miRNA.Results：我们分析了周围的基因表达水平的84 113个已知的miRNAs，有附近的基因，在两个独立的C. elegans表达数据集。与远离这些miRNA的基因相比，在84个miRNA中的59个（71%）附近的基因的表达水平较低。结论：我们发现，在C.秀丽隐杆线虫生殖系miRNA附近种子匹配频率的增加可以部分解释局部效应。
Background: MicroRNAs ( miRNAs) are recently discovered short non-protein-coding RNA molecules. miRNAs are increasingly implicated in tissue-specific transcriptional control and particularly in development. Because there is mounting evidence for the localized component of transcriptional control, we investigated if there is a distance-dependent effect of miRNA.Results: We analyzed gene expression levels around the 84 of 113 know miRNAs for which there are nearby gene that were measured in the data in two independent C. elegans expression data sets. The expression levels are lower for genes in the vicinity of 59 of 84 (71%) miRNAs as compared to genes far from such miRNAs. Analysis of the genes with lower expression in proximity to the miRNAs reveals increased frequency matching of the 7 nucleotide "seed"s of these miRNAs.Conclusion: We found decreased messenger RNA ( mRNA) abundance, localized within a 10 kb of chromosomal distance of some miRNAs, in C. elegans germline. The increased frequency of seed matching near miRNA can explain, in part, the localized effects.