The Dual Antimelanogenic and Antioxidant Activities of the Essential Oil Extracted from the Leaves of Acorus macrospadiceus (Yamamoto) F. N. Wei et Y. K. Li
The Dual Antimelanogenic and Antioxidant Activities of the Essential Oil Extracted from the Leaves of Acorus macrospadiceus (Yamamoto) F. N. Wei et Y. K. Li
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DOI：
10.1155/2012/781280
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发表时间：
2012-01-01
影响因子：
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通讯作者：
Chang, Tsong-Min
中科院分区：
文献类型：
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作者：
Huang, Huey-Chun;Wang, Hsiao-Fen;Chang, Tsong-Min
研究了大苞菖蒲叶挥发油的抗黑色素生成和抗氧化活性。N. Wei et Y. K.李从未被探索过。该精油可有效抑制蘑菇酪氨酸酶活性（EC 50 = 1.57 mg/mL）和B16 F10酪氨酸酶活性（IC 50 = 1.01 mg/mL），降低黑色素含量（EC 50 = 1.04 mg/mL），并降低细胞内活性氧（ROS）水平（EC 50 = 1.87 mg/mL）。该精油能有效清除DPPH（EC 50 = 0.121 mg/mL）和ABTS（+）自由基（EC 50 = 0.122 mg/mL）。它还表现出表观还原力（EC 50 = 0.021 mg/mL）和金属离子螯合活性（EC 50 = 0.029 mg/mL）。挥发油的化学成分为醚类（55.73%）、酮类（19.57%）、单萜类（7.82%）、醇类（3.85%）、酯类（3.77%）、倍半萜类（3.72%）和芳香族化合物（2.85%）。结果证实了A. macrospadiceus精油是一种天然的抗氧化剂和黑色素生成抑制剂。
The antimelanogenic and antioxidant activities of the essential oil extracted from the leaves of Acorus macrospadiceus (Yamamoto) F. N. Wei et Y. K. Li have never been explored. The essential oil effectively inhibited mushroom tyrosinase activity (EC50 = 1.57 mg/mL) and B16F10 tyrosinase activity (IC50 = 1.01 mg/mL), decreased the melanin content (EC50 = 1.04 mg/mL), and depleted the cellular level of the reactive oxygen species (ROS) (EC50 = 1.87 mg/mL). The essential oil effectively scavenged 2,2-diphenyl-1-picryl-hydrazyl (DPPH) (EC50 = 0.121 mg/mL) and 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) ABTS(+) radicals (EC50 = 0.122 mg/mL). It also exhibited an apparent reducing power (EC50 = 0.021 mg/mL) and metal-ion chelating activity (EC50 = 0.029 mg/mL). The chemical constituents of the essential oil are ethers (55.73%), ketones (19.57%), monoterpenes (7.82%), alcohols (3.85%), esters (3.77%), sesquiterpenes (3.72%), and aromatic compounds (2.85%). The results confirm that A. macrospadiceus essential oil is a natural antioxidant and inhibitor of melanogenesis.