Molecular and cellular effects of heat-shock and related treatments of mammalian tissue-culture cells.
Molecular and cellular effects of heat-shock and related treatments of mammalian tissue-culture cells.
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哺乳动物组织培养细胞热休克及相关治疗的分子和细胞效应。
DOI：
10.1101/sqb.1982.046.01.092
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Feramisco,JR
中科院分区：
文献类型：
--
作者：
Thomas,GP;Welch,WJ;Mathews,MB;Feramisco,JR
从原核生物到最复杂的真核生物，大多数(如果不是全部)生物体在其环境受到干扰时都会表现出特定的动态型反应。这些反应从酶功能的暂时性和可逆性改变，到更长期的改变，可能涉及更永久性的基因表达模式的改变，特定的代谢途径被重新定向或阻止。这篇论文的主题是在培养的哺乳动物细胞中诱导一组特定的基因，以响应在高温下或在含有氨基酸类似物的介质中的生长。这些治疗(以及各种其他治疗)会引起我们所说的应激反应，而对于热休克，有证据表明这种反应对细胞具有保护作用(Gerner等人。1976年；麦克阿利斯特和芬克尔斯坦1980年；彼得森和米切尔1981年)。
Most, if not all, organisms, from prokaryotes to the most complex eukaryotes, exhibit specific homeostatic-type responses when their environmental circumstance is perturbed. Such responses range from temporary and reversible modifications of enzyme function, by which specific metabolic pathways are redirected or arrested, to more long-term changes, which may involve altered patterns ofgene expression of a more permanent nature. The subject of this paper is the induction of a particular set of genes in cultured mammalian cells in response to growth at elevated temperatures or in medium containing amino acid analogs. These treatments (as well as a variety of others) give rise to what we refer to as the stress response, and for heat shock there is evidence that the response has a protective effect on cells (Gerner et al. 1976; Mc-Allister and Finkelstein 1980; Petersen and Mitchell 1981).