Simultaneous monitoring of temperature and T1:: Methods and preliminary results of application to drug delivery using thermosensitive liposomes
Simultaneous monitoring of temperature and T1:: Methods and preliminary results of application to drug delivery using thermosensitive liposomes
复制标题
DOI：
10.1002/mrm.20635
复制
发表时间：
2005-10-01
影响因子：
3.3
通讯作者：
Moonen, CTW
中科院分区：
文献类型：
--
作者：
Bos, C;Lepetit-Coiffé, M;Moonen, CTW
提出了一种近实时同时获得温度和纵向弛豫度测量值的方法。定量弛度值由快速Look-Locker EPI数据的信号幅度获得，而恢复曲线上所有信号样本的相位信息结合使用质子共振频率法提供温度值。该技术的实用性在热敏脂质体的体外实验中得到了证明，热敏脂质体被研究为局部药物递送的潜在微载体。该方法允许测量含有顺磁造反差剂的脂质体在rf加热期间的弛豫演化，表明在相变温度附近脂质体的渗透率增加。潜在的应用是使用热敏脂质体监测局部药物输送，并确认在热治疗期间达到脂质体的阈值温度。
A method is presented to obtain temperature and longitudinal relaxivity measurements simultaneously and in near real-time. Quantitative relaxivity values are obtained from the signal magnitude from fast Look-Locker EPI data, whereas phase information from all signal samples on the recovery curve is combined to provide temperature values using the proton resonance frequency method. The utility of this technique is illustrated in an in vitro experiment with thermosensitive liposomes, which are studied as potential micro vehicles for local drug delivery. The method allowed measuring the evolution of relaxivity during RF-heating of liposomes containing a paramagnetic contrast agent, demonstrating increase of liposome permeability near the phase transition temperature. Potential applications are monitoring of local drug delivery using thermosensitive liposomes, and confirmation of reaching the liposomes' threshold temperature during thermal therapy.