hnRNP C Tetramer Measures RNA Length to Classify RNA Polymerase II Transcripts for Export
hnRNP C Tetramer Measures RNA Length to Classify RNA Polymerase II Transcripts for Export
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DOI：
10.1126/science.1218469
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发表时间：
2012-03-30
期刊：
影响因子：
56.9
通讯作者：
Ohno, Mutsuhito
中科院分区：
文献类型：
--
作者：
McCloskey, Asako;Taniguchi, Ichiro;Ohno, Mutsuhito
特定的RNA识别通常是通过特定的RNA序列和/或结构来实现的。然而，我们在这里展示了一种机制，RNA 聚合酶 II (Pol II) 转录本根据其长度进行分类。异质核核糖核蛋白 (hnRNP) C1/C2 的异四聚体通过选择性结合长度超过 200 至 300 个核苷酸的非结构化 RNA 区域，像分子尺一样测量转录本的长度。因此，四聚体将转录物分为两个 RNA 类别，分别作为信使 RNA 或富含尿苷的小核 RNA (U snRNA) 输出，具体取决于它们是否长于阈值。我们的研究结果揭示了 C 四聚体的新功能，并强调了 RNA 长度识别的生物学重要性。
Specific RNA recognition is usually achieved by specific RNA sequences and/or structures. However, we show here a mechanism by which RNA polymerase II (Pol II) transcripts are classified according to their length. The heterotetramer of the heterogeneous nuclear ribonucleoprotein (hnRNP) C1/C2 measures the length of the transcripts like a molecular ruler, by selectively binding to the unstructured RNA regions longer than 200 to 300 nucleotides. Thus, the tetramer sorts the transcripts into two RNA categories, to be exported as either messenger RNA or uridine-rich small nuclear RNA (U snRNA), depending on whether or not they are longer than the threshold, respectively. Our findings reveal a new function of the C tetramer and highlight the biological importance of RNA recognition by the length.