The Effect of Cetylpyridinium Chloride (CPC) on the Cell Surface Hydrophobicity and Adherence of Candida albicansto Human Buccal Epithelial Cells in Vitro
The Effect of Cetylpyridinium Chloride (CPC) on the Cell Surface Hydrophobicity and Adherence of Candida albicansto Human Buccal Epithelial Cells in Vitro
复制标题
氯化十六烷基吡啶（CPC）对体外白色念珠菌对人口腔上皮细胞表面疏水性和粘附的影响
作者：
David S. Jones;L. Schep;M. Shepherd
抽象目的。本研究体外检测了氯化十六烷基吡啶（CPC）对白色念珠菌（MEN 株）细胞表面疏水性（CSH）和芽生孢子对人颊上皮细胞（EEC）粘附的影响。
方法。使用 35SO4 标记的芽生孢子结合 Percoll™ 梯度来确定 CPC 处理白色念珠菌芽生孢子或 BEC 对它们随后粘附的影响。使用疏水相互作用色谱法确定 CPC 处理芽生孢子对其 CSH 的影响。
结果。用 CPC (50 µg mL−1) 处理指数期和稳定期芽生孢子 0.5–30 分钟，或用 CPC (0.5–50 µg mL−1) 处理 15 分钟，可导致体外芽生孢子 CSH 和 BEC 粘附显着降低。用 CPC (0.5–50 µg mL−1) 处理后，CSH 减少和芽生孢子粘附减少之间没有明显相关性 (r < 0.8)。用 CPC (50 µg mL−1) 处理 BEC 0.5–30 分钟或用 CPC (0.5–50 µg mL−1) 处理 15 分钟后，还观察到白色念珠菌（稳定或指数生长期）对人 EEC 的粘附显着降低。在低于和超最低抑制浓度的 CPC 下均观察到抗粘附作用。
结论。这表明，虽然 CPC 降低白色念珠菌 CSH 的能力可能导致其在体外降低对人 BEC 的粘附，但降低的 CSH 只是导致观察到的抗粘附效应的几个可能因素之一。
AbstractPurpose. This study examined the effects of cetylpyridinium chloride (CPC) on cell surface hydrophobicity (CSH) and adherence of blastospores of Candida albicans(MEN strain) to human buccal epithelial cells (EEC) in vitro.
Methods. The effect of CPC treatment of either C. albicans blastospores or BEC on their subsequent adherence was determined using 35SO4 labelled blastospores in association with a Percoll™ gradient. The effects of CPC treatment of blastospores on their CSH was determined using Hydrophobic Interaction Chromatography.
Results. Treatment of exponential and stationary phase blastospores with CPC (50 µg mL−1) for 0.5–30 minutes, or with CPC (0.5–50 µg mL−1) for 15 minutes resulted in significant reductions in both blastospore CSH and adherence to BEC in vitro. No correlation was apparent (r < 0.8) between reduced CSH and reduced blastospore adherence following treatment with CPC (0.5–50 µg mL−1). Significantly reduced adherence of C. albicans (stationary or exponential growth phases) to human EEC was also observed following treatment of BEC with CPC (50 µg mL−1) for 0.5–30 minutes or with CPC (0.5–50 µg mL−1) for 15 minutes. Antiadherence effects were observed at both sub and super-minimum inhibitory concentrations of CPC.
Conclusions. It is suggested that, whilst the ability of CPC to reduce the CSH of C. albicans may contribute to its reduced adherence to human BEC in vitro, reduced CSH is only one of several possible factors that contribute to the observed antiadherence effects.