DETECTION OF ENDOGENOUS MALONDIALDEHYDE-DEOXYGUANOSINE ADDUCTS IN HUMAN LIVER
DETECTION OF ENDOGENOUS MALONDIALDEHYDE-DEOXYGUANOSINE ADDUCTS IN HUMAN LIVER
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DOI：
10.1126/science.8079172
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发表时间：
1994-09-09
期刊：
影响因子：
56.9
通讯作者：
MARNETT, LJ
中科院分区：
文献类型：
--
作者：
CHAUDHARY, AK;NOKUBO, M;MARNETT, LJ
内源性DNA加合物可能与人类遗传性疾病和癌症的病因学有关。内源性DNA加合物的一个潜在来源是脂质过氧化，其产生致突变羰基化合物，如丙二醛。一个敏感的质谱方法允许检测和定量的主要malondiriderde-DNA加合物，一个嘧啶嘌呤酮来自脱氧鸟苷。来自无病人肝的DNA被发现含有5400个加合物/细胞，与外源性致癌物形成的加合物的频率相当。
Endogenous DNA adducts may contribute to the etiology of human genetic disease and cancer. One potential source of endogenous DNA adducts is lipid peroxidation, which generates mutagenic carbonyl compounds such as malondialdehyde. A sensitive mass spectrometric method permitted detection and quantitation of the major malondialdehyde-DNA adduct, a pyrimidopurinone derived from deoxyguanosine. DNA from disease-free human liver was found to contain 5400 adducts per cell, a frequency comparable to that of adducts formed by exogenous carcinogens.