Peripheral blood mitochondrial DNA copy number as a novel potential biomarker for diabetic nephropathy in type 2 diabetes patients
Peripheral blood mitochondrial DNA copy number as a novel potential biomarker for diabetic nephropathy in type 2 diabetes patients
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DOI：
10.3892/etm.2018.6319
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发表时间：
2018-08-01
影响因子：
2.7
通讯作者：
Bakhiet, Moiz
中科院分区：
文献类型：
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作者：
Al-Kafaji, Ghada;Aljadaan, Abdulah;Bakhiet, Moiz
线粒体DNA拷贝数（mtDNA-CN）是线粒体功能的替代指标，改变的mtDNA-CN反映了氧化剂诱导的细胞损伤。我们小组先前的一项研究表明，肾脏mtDNA-CN的减少与糖尿病肾病（DN）的发病机制有关，DN是糖尿病患者终末期肾病的主要原因。在本研究中，它是调查是否在外周血中的mtDNA-CN可用作生物标志物的DN在2型糖尿病（T2 D）患者。该研究包括50名非糖尿病患者和100名糖尿病患者。糖尿病受试者根据其白蛋白与肌酐比值（ACR）分为正常白蛋白尿的T2 D患者（n=50）、微量白蛋白尿的DN患者（n=29）和大量白蛋白尿的DN患者（n=21）。采用实时荧光定量聚合酶链反应（PCR）法检测外周血线粒体DNA-CN。DN组mtDNA-CN低于T2 D组和健康对照组（P
The mitochondrial DNA copy number (mtDNA-CN) is a surrogate measure of mitochondria' function and altered mtDNA-CN reflects the oxidant-induced cell damage. A previous study by our group demonstrated that a reduction in the renal mtDNA-CN is implicated in the pathogenesis of diabetic nephropathy (DN), a leading cause of end-stage renal disease in diabetic patients. In the present study, it was investigated whether the mtDNA-CN in the peripheral blood may be utilized as a biomarker for DN in type 2 diabetes (T2D) patients. The study included 50 non-diabetic and 100 diabetic subjects. The diabetic subjects were sub-divided based on their albumin-to-creatinine ratio (ACR) into T2D patients with normoalbuminuria (n=50), DN patients with microalbuminuria (n=29) and DN patients with macroalbuminuria (n=21). The mtDNA-CN was measured in the peripheral blood by real-time polymerase chain reaction analysis. Patients with DN had a lower mtDNA-CN than patients with T2D and healthy controls (P