Adherence of different Candida dubliniensis isolates in the presence of fluconazole
Adherence of different Candida dubliniensis isolates in the presence of fluconazole
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DOI：
10.1097/00002030-200206140-00005
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发表时间：
2002-06-14
期刊：
影响因子：
3.8
通讯作者：
Tintelnot, K
中科院分区：
文献类型：
--
作者：
Borg-von Zepelin, M;Niederhaus, T;Tintelnot, K
背景：新近报道的酵母菌与白色念珠菌关系密切，主要从HIV感染者和艾滋病患者的口腔中恢复，这些患者经常接受氟康唑预防或治疗口咽念珠菌病。目的：解释都柏林念珠菌在艾滋病患者中日益增加的感染率，并探讨该酵母菌的毒力因素。方法：建立体外实验，比较HIV感染者和白色念珠菌对上皮细胞的黏附能力。结果：所有白色念珠菌的黏附性均好于22株。在氟康唑存在的情况下，与没有氟康唑的对照相比，测试的都柏林弧菌显示出更强的粘附性。相反，所有白色念珠菌分离株在氟康唑存在下与上皮细胞的粘附性降低，与它们对这种药物的体外敏感性无关。蛋白水解酶抗原存在于附着于上皮靶细胞的都柏林隐翅虫细胞表面。在氟康唑存在的情况下，这种蛋白酶抗原的表达更强。结论：在存在氟康唑的情况下，都柏林分支杆菌株的粘附性增加，这可能是近年来从HIV阳性患者中获得较高恢复率的原因。在氟康唑存在的情况下，大多数都柏林螺旋体分离株的蛋白酶分泌被诱导，这可能是粘附性的因素之一。(C)2002年，里平科特·威廉姆斯·威尔金斯。
Background: The recently described yeast species Candida dubliniensis is closely related to C. albicans and has been recovered predominantly from the oral cavities of HIV-infected individuals and AIDS patients who are often receiving fluconazole as prophylactic or therapeutic treatment for oropharyngeal candidiasis. Like C. albicans, C. dubliniensis secretes aspartic poteinases which in C. albicans have been shown to be involved in adherence.Objective: To explain the increasing prevalence of C. dubliniensis in AIDS patients and to investigate the virulence factors of this yeast.Methods: An in vitro assay was developed to compare the adherence to epithelial cells of C. dubliniensis from HIV-patients with that of C. albicans.Results: All C. albicans isolates adhered better than the 22 C. dubliniensis isolates. In the presence of fluconazole, the C. dubliniensis isolates tested showed increased adherence as compared with controls without fluconazole. In contrast, all C. albicans isolates decreased in adherence to epithelial cells in the presence of fluconazole independently of their in vitro susceptibility to this drug. Proteinase antigens are present on the surface of C. dubliniensis cells adherent to epithelial target cells. In the presence of fluconazole this proteinase antigen was more strongly expressed.Conclusion: Increased adherence of C. dubliniensis strains in the presence of fluconazole could explain its high recovery rate from HIV-positive patients in recent years. The induction of proteinase secretion in the presence of fluconazole found for most of the C. dubliniensis isolates could be one of the factors involved in adherence. (C) 2002 Lippincott Williams Wilkins.