Overloading stress-induced progressive degeneration and self-repair in condylar cartilage
Overloading stress-induced progressive degeneration and self-repair in condylar cartilage
复制标题
超载应力诱导髁软骨进行性退化和自我修复
DOI：
10.1111/nyas.14606
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发表时间：
2021-05-07
影响因子：
5.2
通讯作者：
He, Yao
中科院分区：
文献类型：
--
作者：
Fang, Lingli;Ye, Yusi;He, Yao
超负荷应力引起的髁突软骨退变是颞下颌关节骨关节炎（TMJ-OA）的主要病理改变。然而，退化的进展和自我修复的能力仍然知之甚少。在这里，我们使用稳定的张口小鼠模型，通过划分病理阶段来探索软骨退化的进展。然后，我们通过在不同阶段去除载荷来观察软骨的变化，以测试软骨在诱导退变后的潜在自我修复。应用三维共聚焦显微镜结合组织学和显微CT扫描技术，对颞下颌关节自修复前后退变的不同阶段进行观察。结果发现，随着应力超载的发展，软骨发生了进行性的、彻底的退变。在最初的适应阶段，强劲的增殖后内侧软骨开始在该地区的直接负荷。随后，发现广泛的软骨细胞凋亡，随后是新的软骨细胞增殖的聚集体与基质降解和软骨下骨catelation。最后，随着软骨表面损伤，退化达到了一个点，病变不能通过自我修复逆转。术后5天内去除干扰物，软骨基本恢复正常。结果提示，超负荷力作用下，颞下颌关节软骨发生了一系列的退行性变，这种退行性变可以通过早期的自我修复而逆转。
Overloading stress-induced condylar cartilage degeneration acts as the main pathologic change in temporomandibular joint osteoarthritis (TMJ-OA). However, the progression of degeneration and the ability for self-repair remain poorly understood. Here, we explored the progression of cartilage degeneration by dividing pathological stages using a steady mouth-opening mouse model. Then, we observed changes of cartilage by removing the loading at different stages to test the potential self-repair after degeneration induced. Three-dimensional confocal microscopy combined with histology and micro-CT scanning was applied to examine TMJ at different stages of degeneration before and after self-repair. We found the cartilage underwent progressive and thorough degeneration as the overloading stress developed. During the initial adaptation stage, robust proliferation of posteromedial cartilage began at the area of direct loading. Subsequently, widespread chondrocyte apoptosis was found, followed by new chondrocyte proliferation in aggregates with matrix degradation and subchondral bone catabolism. Finally, with cartilage surface damage, the degeneration reached a point where the lesion could not be reversed by self-repair. While the cartilage nearly returned to normal when the interference was removed within 5 days. These results suggested overloading force induces a pathological process of successive degeneration in TMJ cartilage, which can be reversed by self-repair at early stages.