C55 bacteriocin produced by ETB-plasmid positive Staphylococcus aureus strains is a key factor for competition with S. aureus strains.
C55 bacteriocin produced by ETB-plasmid positive Staphylococcus aureus strains is a key factor for competition with S. aureus strains.
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ETB质粒阳性金黄色葡萄球菌菌株产生的C55细菌素是与金黄色葡萄球菌菌株竞争的关键因素。
DOI：
10.1111/1348-0421.12360
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发表时间：
2016
期刊：
影响因子：
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通讯作者：
Komatsuzawa H
中科院分区：
文献类型：
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作者：
Kawada-Matsuo M;Shammi F;Oogai Y;Nakamura N;Sugai M;Komatsuzawa H
金黄色葡萄球菌产生的表皮剥脱毒素（ET）与大疱性脓疱病的发病密切相关。到目前为止，已经确定了三个潜在危险（ETA、ETB和ETD）。编码ETB的基因位于命名为pETB的质粒中。细菌素合成基因也位于该质粒中，据报道pETB阳性菌株产生C55细菌素。本实验研究了其对S.研究了pETB阳性菌株TY 4产生的细菌素的金黄色葡萄球菌菌株。该细菌素对所有pETB阴性菌株（包括TY 4）均具有抗菌活性，但对pETB阳性菌株无抗菌活性。此外，删除了pETB质粒的TY 4 −菌株显示出对细菌素的敏感性。进一步的实验表明，pETB质粒中细菌素合成基因下游的两个免疫因子（orf 46 - 47和orf 48）与针对TY 4产生的细菌素的免疫力相关。TY 4 − withorf 46 - 47菌株表现出对细菌素的完全抗性，而TY 4 − withorf 48菌株表现出部分抗性。细菌素是否影响与S. aureusstrains也进行了研究。当TY 4或TY 4 −与对细菌素敏感的209 P菌株共培养时，209 P与TY 4共培养的比例显著低于209 P与TY 4 −共培养时的比例，而TY 4 −与46 - 48共培养的比例大于TY 4 −。这些结果表明，当pETB阳性和阴性菌株共存时，pETB阳性菌株产生的C55细菌素影响每种菌株的比例。
Exfoliative toxin (ET) produced byStaphylococcusaureusis closely associated with the onset of bullous impetigo. To date, three ETs (ETA, ETB and ETD) have been identified. The gene encoding ETB is located in a plasmid designated pETB. Bacteriocin synthesis genes are also located in this plasmid and pETB‐positive strains reportedly produce the C55 bacteriocin. In this study, the antibacterial activity againstS. aureusstrains of the bacteriocin produced by the pETB‐positive strain TY4 was investigated. This bacteriocin demonstrated antibacterial activity against all pETB‐negative but not pETB‐positive strains, including TY4. Additionally, a TY4− strain from which the pETB plasmid had been deleted exhibited susceptibility to the bacteriocin. Further experiments revealed that two immunity factors (orf46‐47 andorf48) downstream of the bacteriocin synthesis genes in the pETB plasmid are associated with immunity against the bacteriocin produced by TY4. The TY4− withorf46‐47strain exhibited complete resistance to bacteriocin, whereas the TY4− withorf48strain exhibited partial resistance. Whether bacteriocin affects the proportion of each strain when co‐cultured withS. aureusstrains was also investigated. When TY4 or TY4− was co‐cultured with 209P strain, which is susceptible to the bacteriocin, the proportion of 209P co‐cultured with TY4 was significantly less than when 209P was co‐cultured with TY4−, whereas the proportion of TY4− withorf46‐48co‐cultured with TY4 was greater than with TY4−. These results suggest that the C55 bacteriocin produced by pETB‐positive strains affects the proportion of each strain when pETB‐positive and ‐negative strains co‐exist.