Development of Gateway Binary Vectors, R4L1pGWBs, for Promoter Analysis in Higher Plants
Development of Gateway Binary Vectors, R4L1pGWBs, for Promoter Analysis in Higher Plants
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DOI：
10.1271/bbb.90720
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发表时间：
2009-11-01
影响因子：
1.6
通讯作者：
Nakagawa, Tsuyoshi
中科院分区：
文献类型：
--
作者：
Nakamura, Shinya;Nakao, Akihide;Nakagawa, Tsuyoshi
我们开发了一系列用于植物转化的Gateway双元载体R4 L1 pGWB，其允许容易地构建启动子：报告基因克隆。R4 L1 pGWB含有重组attR 4-attL 1-报告基因盒，因此通过Gateway LR反应有效地掺入attL 4-启动子-attR 1进入克隆，从而产生attB 4-启动子-attR 1-报告基因构建体。在R4 L1 pGWB中使用的报告基因是P-葡萄糖醛酸酶（GUS）、荧光素酶（LUC）、增强型黄色荧光蛋白（EYFP）、增强型青色荧光蛋白（ECFP）、G3绿色荧光蛋白（G3 GFP）、G3 GFP-GUS和标记红色荧光蛋白（TagRFP）。
We developed a new series of Gateway binary vectors for plant transformation, R4L1pGWBs, which allow easy construction of promoter: reporter clones. R4L1pGWBs contain a recombination attR4-attL1-reporter cassette, and thus an attL4-promoter-attR1 entry clone was efficiently incorporated by the Gateway LR reaction, resulting in the generation of an attB4-promoterallB1-reporter construct. The reporters employed in R4L1pGWBs were P-glucuronidase (GUS), luciferase (LUC), enhanced yellow fluorescent protein (EYFP), enhanced cyan fluorescent protein (ECFP), G3 green fluorescent protein (G3GFP), G3GFP-GUS, and tag red fluorescent protein (TagRFP).