Oviduct-Embryo Interactions in Cattle: Two-Way Traffic or a One-Way Street?
Oviduct-Embryo Interactions in Cattle: Two-Way Traffic or a One-Way Street?
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DOI：
10.1095/biolreprod.115.127969
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发表时间：
2015-06-01
影响因子：
3.6
通讯作者：
Rizos, Dimitrios
中科院分区：
文献类型：
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作者：
Maillo, Veronica;Gaora, Peadar O.;Rizos, Dimitrios
本研究检测了单个或多个胚胎的存在对牛输卵管转录组的影响。在试验1中，发情后第3天屠宰发情期（未配种，n = 6）和妊娠期（人工授精，n = 11）小母牛，分别冲洗黄体同侧输卵管的壶腹和峡部。将来自峡部区域的输卵管上皮细胞（所有卵母细胞/胚胎都位于峡部区域中）快速冷冻用于微阵列分析。在实验2中，小母牛被分为周期（nonbred，n = 6）或怀孕（多个胚胎移植，n = 10）组。在动情后1.5天，通过内窥镜将体外产生的推定受精卵转移到同侧输卵管（n = 50个受精卵/小母牛）。在第3天屠宰小母牛，回收输卵管峡部上皮细胞用于RNA测序。实验1中的微阵列分析未能检测到由单个胚胎的存在诱导的输卵管峡部的转录组的任何差异。在实验2中，多个胚胎移植后，RNA测序显示278个差异表达基因，其中123个上调，155个下调，在怀孕的小母牛。下调的基因多与免疫功能有关。总之，输卵管中存在多个胚胎导致在输卵管峡部检测到差异表达的基因;在单个胚胎存在的情况下未能检测到输卵管转录组的变化可能是由于在本研究的条件下局部和不可检测的影响。
This study examined the effect of the presence of single or multiple embryos on the transcriptome of the bovine oviduct. In experiment 1, cyclic (nonbred, n = 6) and pregnant (artificially inseminated, n = 11) heifers were slaughtered on Day 3 after estrus, and the ampulla and isthmic regions of the oviduct ipsilateral to the corpus luteum were separately flushed. Oviductal epithelial cells from the isthmus region, in which all oocytes/embryos were located, were snap-frozen for microarray analysis. In experiment 2, heifers were divided into cyclic (nonbred, n = 6) or pregnant (multiple embryo transfer, n = 10) groups. In vitro-produced presumptive zygotes were transferred endoscopically to the ipsilateral oviduct on Day 1.5 postestrus (n = 50 zygotes/heifer). Heifers were slaughtered on Day 3, and oviductal isthmus epithelial cells were recovered for RNA sequencing. Microarray analysis in experiment 1 failed to detect any difference in the transcriptome of the oviductal isthmus induced by the presence of a single embryo. In experiment 2, following multiple embryo transfer, RNA sequencing revealed 278 differentially expressed genes, of which 123 were up-regulated and 155 were down-regulated in pregnant heifers. Most of the down-regulated genes were related to immune function. In conclusion, the presence of multiple embryos in the oviduct resulted in the detection of differentially expressed genes in the oviductal isthmus; failure to detect changes in the oviduct transcriptome in the presence of a single embryo may be due to the effect being local and undetectable under the conditions of this study.