Gene manipulation by homologous recombination in Escherichia coli.
Gene manipulation by homologous recombination in Escherichia coli.
复制标题
在大肠杆菌中通过同源重组进行基因操作。
DOI：
10.1089/dna.1988.7.729
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
McEwan,RN
中科院分区：
文献类型：
--
作者：
Crouse,GF;McEwan,RN
The use of homologous recombination inEscherichia coliis described as a tool for DNA manipulation. The utility of the method is illustrated by the addition of 3′-flanking sequences to adhfrminigene by plasmid-phage recombination involving asupF-containingdhfrminigene plasmid and a λCharon4A phage containing the 3′ end of thedhfrgene. In addition, other uses of both plasmid-phage and phage-phage recombination in gene manipulation are described.
